Millipore Sigma Vibrant Logo

MABF397 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5

View Products on Sigmaaldrich.com
MABF397
100 µg  
Preis wird abgerufen...
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
Bitte erfragen
Eingeschränkte Verfügbarkeit
Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Sonderaktionen

       

      Kontakt zum Kundenservice

      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIP, IHC, FCRPE-Cy7Monoclonal Antibody
      Description
      Catalogue NumberMABF397
      DescriptionAnti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5
      Alternate Names
      • Interleukin-2 receptor subunit alpha
      • IL-2 receptor subunit alpha
      • IL-2-RA
      • IL-2R subunit alpha
      • IL2-RA
      • p55
      • CD25
      Background InformationThe PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
      References
      Product Information
      FormatPE-Cy7
      PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.
      Key Applications
      • Immunoprecipitation
      • Immunohistochemistry
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      ClonePC61.5
      Concentration0.2 mg/mL
      HostRat
      IsotypeIgG1λ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Il2ra
      • Il2r
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABF397 04053252959899

      Verwandte Produkte & Anwendungen

      Related Products

      Bestellnummer Beschreibung  
      MABF396 Anti-CD25 Antibody (mouse), APC, clone PC61.5 Preis & Verfügbarkeit
      MABF548 Anti-CD25 Antibody (mouse), APC, clone PC61.5 Preis & Verfügbarkeit
      MABF549 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5 Preis & Verfügbarkeit
      MABF550 Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5 Preis & Verfügbarkeit

      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies