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AB3403-I Anti-Beta Galactosidase Antibody, E. coli

Anti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.

Anti-Beta Galactosidase Antibody, E. coli: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: Epitope Tags Gene Symbol: GLB1, ELNR1 UniProt Number: P16278
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AB3403-I
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BactWBChAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3403-I
      ReplacesAB3403
      DescriptionAnti-Beta Galactosidase Antibody, E. coli
      Alternate Names
      • Beta-galactosidase
      • Acid beta-galactosidase
      • Lactase
      • Elastin receptor 1
      Background Informationβ Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein corresponding to E. Coli Beta Galactosidase.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostChicken
      Species Reactivity
      • Bacteria
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GLB1
      • ELNR1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~120 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in TOP10/PCU19 E. Coli lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB3403-I 04053252941399

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies