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ABF1051-100UL Anti-Adenovirus 5 Packaging protein IVa2

Anti-Adenovirus 5 Packaging protein IVa2, Cat. No. ABF1051, is a rabbit polyclonal antibody that detects Adenovirus 5 Packaging protein IVa2 and is tested for use in Western Blotting and Immunofluorescence.

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100 µL  
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      Replacement Information
      Description
      Catalogue NumberABF1051-100UL
      DescriptionAnti-Adenovirus 5 Packaging protein IVa2
      Alternate Names
      • Packaging protein 1
      Background InformationPackaging protein 1 (UniProt: P03271; also known as Packaging protein IVa2) is encoded by the IVa2 gene (Gene ID: 5739974) in human. Adenovirus 5. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. Packaging protein IVa2 is a multifunctional, homodimeric, protein that is expressed in the intermediate phase of the viral replicative cycle. It is detected in both empty and mature virus particles. It is a component of the packaging machinery that encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter. Its DNA-binding region is localized in amino acids 440-449. It binds, along with packaging proteins 2 and 3, to the specific packaging sequence on the left end of viral genomic DNA and displays ATPase activity thereby providing the power stroke of the packaging machinery. Its activity is reported to be stimulated by protein 33K that acts as a terminase. It is shown to specifically bind to the 5'-CG-3' nucleotides of the repeats making up the packaging sequence. (Ref.: Charman, M., et al. (2023). Nature. 616(7956):332-338; Pérez-Berná, AJ., et al. (2014). J. Virol. 88(3); 1513-1524; Ostapchuk, P., et al. (2005). J. Virol. 79(5); 2831-2838).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antisera without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Adenovirus 5 Packaging protein IVa2, Cat. No. ABF1051, is a rabbit polyclonal antibody that detects Adenovirus 5 Packaging protein IVa2 and is tested for use in Western Blotting and Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected Adenovirus Packaging protein IVa2 in Immunofluorescence application. (Charman, M., et al. (2023). Nature. 616(7956):332-338).

      Western Blotting Analysis: A representative lot detected Adenovirus Packaging protein IVa2 in Western Blotting application. (Subramanian, T., et al. (2006). J. Virol. 80(4); 2000-2012).

      Western Blotting Analysis: A 1:2,500 dilution from a representative lot detected Adenovirus 5 Packaging protein IVa2 in HeLa cells infected with Human adenovirus type 5. (Courtesy of Dr. Patrick Hearing, Stony Brook University).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged, full-length, recombinant Packaging protein IVa2 from human adenovirus 5.
      EpitopeUnknown
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Packaging protein IVa2 of Human Adenovirus 5.
      IsotypeIgGκ
      Species Reactivity
      • Virus
      Species Reactivity NoteAdenovirus 5.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IVa2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~51 kDa observed; 50.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells infected Human adenovirus type 5.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adenovirus 5 Packaging protein IVa2 in lysate from HeLa cells infected with human adenovirus type 5, but not in lysate from uninfected cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABF1051-100UL 04065271174900