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MAB348-AF555 Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate is an antibody against APP A4 for use in Immunocytochemistry.

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Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, A4, AD1 UniProt Number: P05067
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MAB348-AF555
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      F, Ca, H, M, R, Porcine, MkICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue NumberMAB348-AF555
      DescriptionAnti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate
      Alternate Names
      • Amyloid beta A4 protein
      • ABPP
      • Alzheimer disease amyloid protein
      • Amyloid precursor protein
      • APP
      • APPI
      • Cerebral vascular amyloid peptide
      • CVAP
      • PN-II
      • PreA4
      • Protease nexin-II
      • PN-II
      • N-APP
      • Soluble APP-alpha
      • S-APP-alpha
      • Soluble APP-beta
      • S-APP-beta
      • C99
      • Beta-amyloid protein 42
      • Beta-APP42
      • Beta-amyloid protein 40
      • Beta-APP40
      • C83
      • P3(42)
      • P3(40)
      • C80
      • Gamma-secretase C-terminal fragment 59
      • Amyloid intracellular domain 59
      • AICD-59
      • AID(59)
      • Gamma-CTF(59)
      • Gamma-secretase C-terminal fragment 57
      • Amyloid intracellular domain 57
      • AICD-57
      • AID(57)
      • Gamma-CTF(57)
      • Gamma-secretase C-terminal fragment 50
      • Amyloid intracellular domain 50
      • AICD-50
      • AID(50)
      • Gamma-CTF(50)
      • C31
      Background InformationAmyloid beta A4 protein (UniProt P05067; also known as ABPP, Alzheimer disease amyloid protein, Amyloid precursor protein, APP, APPI, Cerebral vascular amyloid peptide, CVAP, PN-II, PreA4, Protease nexin-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID 351) in human. Amyloid precursor protein (APP) is initially produced with a signal peptide sequence (a.a. 1-17), the removal of which yields the mature protein with a large extracellular portion (a.a. 18-699), followed by a transmembrane segment (a.a. 700-723) and a cytoplasmic (a.a. 724-770) tail. APP can be further processed by the α-, β-, and γ-secretases in two alternative processing pathways. In the non-amyloidogenic pathway, APP is first cleaved by the plasma membrane-localized α-secretase to generate an N-terminal extracellular sAPPα fragment (a.a. 18-687) and a membrane-bound C-terminal fragment C83 (CTFα), which can be further cleaved by γ-secretase to produce a non-toxic small peptide p3 and a cytoplasmic APP intracellular domain (AICD). In the amyloidogenic pathway, APP undergoes β-cleavage in BACE-1 (β-site APP-cleaving enzyme)-enriched endosomes to generate an N-terminal extracellular sAPPβ fragment (a.a. 18-671) and a membrane-bound C-terminal fragment C99 (CTFβ). Subsequent cleavage of C99 by γ-secretase releases the amyloid β peptide (Aβ) and AICD. Aβ accumulation in the cortical and hippocampal regions of the brain is a major pathological feature of Alzheimer's disease (AD).
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate is an antibody against APP A4 for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. MAB348) is shown to be suitable also for immunohistochemistry (for both frozen and paraffin-embedded sections), immunofluorescence, and Western blotting applications.
      Biological Information
      ImmunogenPurified recombinant Alzheimer precursor A4 (pre-A4695) fusion protein.
      EpitopeAmino acids 66-81 of APP.
      Clone22C11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 22C11 reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich, C., et al. (1993). J. Biol. Chem. 268(35):26571-26577). Clone 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann, J., et al. (2000). Eur. J. Cell. Biol.79(12):905-914.). The antibody is known to cross react with APLP2 (Slunt, H.H., et al. (1994). J. Biol. Chem. 269(4):2637-2644).
      IsotypeIgG1
      Species Reactivity
      • Fish
      • Canine
      • Human
      • Mouse
      • Rat
      • Porcine
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~110/120/130 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in SH-5YSY cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP A4 in SH-5YSY cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MAB348-AF555 04055977311976

      Documentation

      Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate Analysenzertifikate

      TitelChargennummer
      Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 - 3425426 3425426
      Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 - 4083057 4083057
      Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 -Q2637233 Q2637233
      Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 Monoclonal Antibody 2926714
      Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 Monoclonal Antibody 3111121

      Broschüre

      Titel
      Neuroscience Solutions for productive research