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ST1558 Anti-ALOX12 Mouse mAb (2D10)

Anti-ALOX12, mouse monoclonal, clone 2D10, recognizes the ALOX12 protein. It is validated for ELISA and Western blotting.

Anti-ALOX12 Mouse mAb (2D10): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Arachidonate 12-Lipoxygenase

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ST1558
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Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Preis & Verfügbarkeit

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ST1558-100UG
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      		 100 μg
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      Description
      OverviewRecognizes the ALOX12 protein.
      Catalogue NumberST1558
      Brand Family Calbiochem®
      SynonymsAnti-Arachidonate 12-Lipoxygenase
      Application Data
      Detection of human ALOX12 by immunoblotting. Sample: Partial, recombinant, human ALOX12 protein. Primary antibody: Anti-ALOX12 Mouse mAb (2D10) (Cat. No. ST1558) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 564-664 of human ALOX12, expressed as a GST fusion protein
      Clone2D10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1558-100UG 04055977224283

      Documentation

      Anti-ALOX12 Mouse mAb (2D10) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-Arachidonate 12-Lipoxygenase
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human ALOX12 by immunoblotting. Sample: Partial, recombinant, human ALOX12 protein. Primary antibody: Anti-ALOX12 Mouse mAb (2D10) (Cat. No. ST1558) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ALOX12 protein.
      BackgroundLipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1 and 15-LOX-2, which catalyze the regio-selective addition of oxygen into arachidonic acid, and epidermal LOX (E-LOX) which metabolizes 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) to a specific epoxyalcohol compound. Different isozymes have different physiological roles; 5-, 12R-, 12S- and 15-LOX-1 have proliferative, antiapoptotic and angiogenic functions whilst 15-LOX-2 has roles in differentiation, growth arrest and the induction of apoptosis. E-LOX, the most divergent member of this family, is involved in terminal differentiation of keratinocytes and has a major role in skin barrier function. Perturbations in lipoxygenase enzymes have been implicated in various human diseases including bronchial asthma, atherosclerosis, osteoporosis and some cancers.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 564-664 of human ALOX12, expressed as a GST fusion protein
      Clone2D10
      IsotypeIgG2a
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review