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ABS102 Anti-AKAP 79 Antibody

Detect AKAP 79 using this Anti-AKAP 79 Antibody validated for use in WB.

Anti-AKAP 79 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: AKAP5, AKAP79 UniProt Number: P24588
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ABS102
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS102
      DescriptionAnti-AKAP 79 Antibody
      Alternate Names
      • A-kinase anchor protein 5
      • AKAP-5
      • A-kinase anchor protein 79 kDa
      • AKAP 79
      • H21
      • cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
      Background InformationA-kinase anchoring protein 79 (AKAP 79) is a scaffolding protein that resides predominantly in excitatory neuronal synapses. AKAP 79 interacts with membrane-associated guanylate kinase (MAGUK) to be used by glutamate receptors. AKAP 79 may also be involved with SAP97 and other large signaling complexes. AKAP 79 is essential for forskolin-induced up-regulation of L-current.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect AKAP 79 using this Anti-AKAP 79 Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human AKAP 79.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes AKAP 79 at the C-terminus.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq].
      Gene Symbol
      • AKAP5
      • AKAP79
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.

      SUBUNIT STRUCTURE: Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor.

      SUBCELLULAR LOCATION: Membrane. Note: Associated with particulate fractions.

      DOMAIN: RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

      MISCELLANEOUS: The N-terminal region, which is highly basic, is required for interaction with calmodulin.

      SEQUENCE SIMILARITIES: Contains 1 AKAP domain.
      Molecular Weight~79 kDa observed.
      AKAP79 migrates in WB with an apparent molecular weight of ~79 kDa. Uncharacterized bands appear at ~35 kDa and ~13 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79.

      Western Blot Analysis: 1 µg/mL of this antibody detected AKAP 79 on 10 µg of HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABS102 04053252420306