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176905 Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis - Calbiochem

Anthrax Protective Antigen, Bacillus anthracis, Recombinant, is the protective antigen that mediates the entry of anthrax lethal factor and edema factor into the target cell.

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      Übersicht

      Replacement Information
      Description
      OverviewRecombinant, Bacillus anthracis anthrax protective antigen expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, protective antigen (PA) is the central moiety that mediates the entry of lethal factor and edema factor into the target cell. PA binds to the cell surface via a type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor. After proteolytic activation to a 63 kDa fragment, PA self-associates to form a heptameric cation-selective, voltage-gated membrane channel.
      Catalogue Number176905
      Brand Family Calbiochem®
      References
      ReferencesMogridge, J., et al. 2002. Biochemistry 41, 1079.
      Nassi, S., et al. 2002. Biochemistry 41, 1445.
      Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun. 286, 6.
      Bradley, K.A., et al. 2001. Nature 414, 225.
      Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.
      Product Information
      ATP CompetitiveN
      FormLyophilized
      FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
      ReversibleN
      Quality LevelMQ100
      Applications
      ApplicationAnthrax Protective Antigen, Bacillus anthracis, Recombinant, is the protective antigen that mediates the entry of anthrax lethal factor and edema factor into the target cell.
      Biological Information
      Primary TargetType I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor
      PuritySingle major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
      End use certificateY
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      176905 0

      Documentation

      Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis - Calbiochem Analysenzertifikate

      TitelChargennummer
      176905

      Literatur

      Übersicht
      Mogridge, J., et al. 2002. Biochemistry 41, 1079.
      Nassi, S., et al. 2002. Biochemistry 41, 1445.
      Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun. 286, 6.
      Bradley, K.A., et al. 2001. Nature 414, 225.
      Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-April-2008 RFH
      DescriptionRecombinant, Bacillus anthracis anthrax protective antigen expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Central moiety that mediates entry of lethal factor (LF) and edema factor (EF) into the target cell. PA83 binds to the cell surface via a type I membrane receptor with a von Willebrand factor A domain, known as anthrax toxin receptor. PA83 is proteolytically activated in vivo by a furin-like protease to produce a 63 kDa protein (PA63). PA63 self-associates to form a heptameric, cation-selective, voltage-gated, membrane channel that subsequently binds to LF and/or EF. Cleavage of the 20 kDa fragment from PA83 is necessary for binding of protective antigen to LF and EF.
      FormLyophilized
      FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
      PuritySingle major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.
      SolubilityReconstitute in 100 µl sterile, distilled H₂O to yield a final concentration of 1 mg/ml.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesMogridge, J., et al. 2002. Biochemistry 41, 1079.
      Nassi, S., et al. 2002. Biochemistry 41, 1445.
      Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun. 286, 6.
      Bradley, K.A., et al. 2001. Nature 414, 225.
      Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.