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HLINJMAG-75K MILLIPLEX MAP Human Liver Injury Magnetic Bead Panel - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), α-glutathianone S-transferase (GSTα), malate dehydrogenase 1 (MDH1), sorbitol dehydrogenase (SDH), and 5'-Nucleotidase

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Toxicity Research Sub-Category: Toxicity
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HLINJMAG-75K
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Descripción

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ARG1 GSTα SDH 5'-NT/CD73 Malate dehydrogenase 1 (MDH1) HMplexLuminex xMAP
Description
Catalogue NumberHLINJMAG-75K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Liver Injury Magnetic Bead Panel - Toxicity Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Liver Injury Panel contains all the components necessary to simultaneous quantify the following 5 analytes in serum and plasma samples:

• Liver-Type Arginase 1 (ARG1)*
• Malate dehydrogenase 1 (MDH1)*
• α-glutathione S-transferase (GSTα)*
• Sorbitol Dehydrogenase (SDH)*
• 5'-Nucleotidase/CD73 (5’-NT)

*ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).
Background InformationDrug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development.

The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione Stransferase (GSTα), Malate dehydrogenase 1 (MDH1) and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. 5'-Nucleotidase/CD73 (5’-NT) is a traditional biomarker recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA).
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <20
  • Intra-assay: <10
Panel TypeMAGNETIC Toxicity
Quality LevelMQ100
Applications
ApplicationThe analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), α-glutathianone S-transferase (GSTα), malate dehydrogenase 1 (MDH1), sorbitol dehydrogenase (SDH), and 5'-Nucleotidase (5'-NT/CD73).
Key Applications
  • Multiplexing
Application Notes• This is a same day assay.
• This assay uses 25uL of diluted (1:5) serum or plasma samples. Tissue culture supernatants may require dilution, as well. A two hour or overnight incubation can be used.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Human
Analytes Available
  • ARG1
  • GSTα
  • SDH
  • 5'-NT/CD73
  • Malate dehydrogenase 1 (MDH1)
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 106% - 123%
Physicochemical Information
Sensitivity
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • ARG1: 69-50,000 pg/mL
  • MDH1: 69-50,000 pg/mL
  • GSTα: 14-10,000 pg/mL
  • SDH: 549-400,000 pg/mL
  • 5'-NT/CD73: 41-30,000 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Global Trade Item Number
Número de referencia GTIN
HLINJMAG-75K 04054839177613