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402176 ImmunoPen™

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402176
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402176-1EA
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      Description
      OverviewDesigned to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
      Catalogue Number402176
      Brand Family Calbiochem®
      Product Information
      DeclarationNot available for sale in Japan.
      Applications
      Application NotesFrozen Sections
      Immunostaining Barrier
      Paraffin Sections
      Application CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

      Paraffin Section Method:
      Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
      Frozen Section Method:
      Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
      Global Trade Item Number
      Número de referencia GTIN
      402176-1EA 07790788054199