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400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem

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Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Primary Target: growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
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400051
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Replacement Information

Tabla espec. clave

CAS #Empirical Formula
31282-04-9C₂₀H₃₇N₃O₁₃

Precios y disponibilidad

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      Description
      OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
      Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
      Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
      Catalogue Number400051
      Brand Family Calbiochem®
      References
      ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
      Hamada, W., et al. 1994. Curr. Genetics 26, 251.
      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
      Sugimoto, K., et al. 1994. Plant J. 5, 863.
      Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
      Gaken, J., et al. 1992. Biotechniques 13, 32.
      Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
      Ma, H., et al. 1992. Gene 117, 161.
      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
      Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
      Bilang, R., et al. 1991. Gene 100, 247.
      Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
      Zhou, J., et al. 1991. Gene 107, 307.
      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
      Kronstad, J.W., et al. 1989. Gene 79, 97.
      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
      Cullen, D., et al. 1987. Gene 57, 21.
      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
      Product Information
      CAS number31282-04-9
      FormAmber to brown liquid
      Hill FormulaC₂₀H₃₇N₃O₁₃
      Chemical formulaC₂₀H₃₇N₃O₁₃
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
      Primary Targetgrowth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
      Secondary targetprotein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
      Purity>85% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSWK2130000
      Safety Information
      R PhraseR: 26/27/28-37/38-41

      Very toxic by inhalation, in contact with skin and if swallowed.
      Irritating to respiratory system and skin.
      Risk of serious damage to eyes.
      S PhraseS: 23-26-36/37/39-45

      Do not breathe fumes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic & Carcinogenic / Teratogenic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

      Note: This product is highly toxic. Read enclosed MSDS before handling.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      400051-5MU 07790788049966

      Documentation

      Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem Certificados de análisis

      CargoNúmero de lote
      400051

      Referencias bibliográficas

      Visión general referencias
      Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
      Hamada, W., et al. 1994. Curr. Genetics 26, 251.
      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
      Sugimoto, K., et al. 1994. Plant J. 5, 863.
      Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
      Gaken, J., et al. 1992. Biotechniques 13, 32.
      Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
      Ma, H., et al. 1992. Gene 117, 161.
      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
      Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
      Bilang, R., et al. 1991. Gene 100, 247.
      Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
      Zhou, J., et al. 1991. Gene 107, 307.
      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
      Kronstad, J.W., et al. 1989. Gene 79, 97.
      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
      Cullen, D., et al. 1987. Gene 57, 21.
      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

      Folleto

      Cargo
      Antibiotics Profiler
      Bulk Product Guide
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-January-2018 JSW
      DescriptionHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).

      Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).

      The analytical data listed below will vary from lot to lot.

      Bioassay: 350-450 U/mg
      Concentration: 444,600 U/ml; 394 mg Hygromycin B/ml
      Density: 1140-1155 mg/ml
      % solids: 34.6%
      Fill volumes:
      100,000 U = 225 µl
      1,000,000 U = 2.25 ml
      10,000,000 U = 22.5 ml
      250,000 U = 562 µl
      5,000,000 U = 11.25 ml
      FormAmber to brown liquid
      Recommended reaction conditions
      Hygromycin B is not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 µm filter before use. When using this product for mammalian cell selection the working concentration is generally between 50 µg/ml and 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type. For researchers interested in understanding how the concentration in mg/ml can be calculated from the activity in U/ml we provide the following example as a guideline. For the 1,000,000 Unit size: 1,000,000 Units at 438,440 U/ml

      Figure 1: Calculations
      CAS number31282-04-9
      RTECSWK2130000
      Chemical formulaC₂₀H₃₇N₃O₁₃
      Structure formulaStructure formula
      Purity>85% by HPLC
      Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

      Note: This product is highly toxic. Read enclosed MSDS before handling.
      Toxicity Highly Toxic & Carcinogenic / Teratogenic
      Merck USA index14, 4852
      ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
      Hamada, W., et al. 1994. Curr. Genetics 26, 251.
      Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
      Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
      Sugimoto, K., et al. 1994. Plant J. 5, 863.
      Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
      Gaken, J., et al. 1992. Biotechniques 13, 32.
      Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
      Ma, H., et al. 1992. Gene 117, 161.
      MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
      Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
      Bilang, R., et al. 1991. Gene 100, 247.
      Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
      Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
      Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
      MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
      Zhou, J., et al. 1991. Gene 107, 307.
      Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
      Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
      Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
      Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
      Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
      Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
      Kronstad, J.W., et al. 1989. Gene 79, 97.
      Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
      Cullen, D., et al. 1987. Gene 57, 21.
      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
      Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.