Separation of chlorogenic acid in apple juice

Column Purospher® STAR RP-18 endcapped (5μm) LiChroCART® 205-4,6 HPLC column (150252)
Precolumn Purospher® STAR RP-18 endcapped (5μm) LiChroCART® 4-4 (150250)
Oven temperature 40°C
Flow Rate 1 mL /min
Mobile Phase A 0.1% Phosphoric acid (1 mL 85% Phosphoric acid in to 1 L with HPLC Water)
Mobile Phase B Acetonitrile
GradientTime (min)Eluent AEluent B
0.0
1.4
30.5
33.5
41.8
51.9
55.0
57.0
60.0
65.0
85.1		 100
94
88
83
81
37
37
0
0
100
100		 0
6
12
17
19
63
63
100
100
0
0
Sample Fresh apple juice
Peak Chlorogenic acid