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324682 Elastase, Porcine Pancreas, High Purity, Crystallized

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324682
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324682-1000U
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      Description
      OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
      Catalogue Number324682
      Brand Family Calbiochem®
      References
      ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
      Mandl, I. 1962. Methods in Enzymol. 5, 665.
      Product Information
      CAS number39445-21-1
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
      EC number3.4.21.36
      FormLyophilized
      FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
      Quality LevelMQ100
      Biological Information
      Specific Activity≥25 units/mg protein
      Physicochemical Information
      ContaminantsChymotrypsin: ≤0.2%
      Safety Information according to GHS
      RTECSTS5052000
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
      Global Trade Item Number
      Catalogue Number GTIN
      324682-1000U 04055977196832