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324681 Elastase, Human Neutrophil

Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans

Elastase, Human Neutrophil MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PMN Elastase

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324681
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      Description
      OverviewNative elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.
      Catalogue Number324681
      Brand Family Calbiochem®
      SynonymsPMN Elastase
      References
      ReferencesSiedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
      Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
      Selak, M.A. 1992. Thromb. Haemost. 68, 570.
      Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
      Product Information
      CAS number9004-06-2
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=454454">Cat. No. 454454</a>) per min at 25°C, pH 8.0.
      EC number3.4.21.37
      FormLyophilized solid
      FormulationSalt-free.
      Quality LevelMQ200
      Applications
      Biological Information
      PurityMajor band by SDS-PAGE
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity≥20 units/mg protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSJX7069000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      324681-100UG 07790788059583

      Documentation

      Elastase, Human Neutrophil SDS

      Title

      Safety Data Sheet (SDS) 

      Elastase, Human Neutrophil Certificates of Analysis

      TitleLot Number
      324681

      References

      Reference overview
      Siedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
      Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
      Selak, M.A. 1992. Thromb. Haemost. 68, 570.
      Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.

      Citations

      Title
    • Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.
    • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
    • Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-May-2008 RFH
      SynonymsPMN Elastase
      DescriptionNative elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.
      FormLyophilized solid
      FormulationSalt-free.
      Recommended reaction conditions
      Human Neutrophil Elastase Protocol 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val-pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an ε of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number9004-06-2
      RTECSJX7069000
      EC number3.4.21.37
      PurityMajor band by SDS-PAGE
      Specific activity≥20 units/mg protein.
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
      SolubilityReconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 3535
      ReferencesSiedle, B., et al. 2002. Biorg. Med. Chem. 10, 2855.
      Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
      Selak, M.A. 1992. Thromb. Haemost. 68, 570.
      Baugh, R.J., and Travis, J. 1976. Biochemistry 15, 836.
      Citation
    • Tianbo Li and Hans G. Folkesson. (2005) RNA interference for αENaC inhibits rat lung fluid absorption in vivo. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L649-L660.
    • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
    • Valerie A. Fadok, et al. (2001) Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. Journal of Immunology 166, 6847-6854.
      		•