Direct Detect^® Spectrometer

What if you never had to run another Bradford or BCA, ever? The Direct Detect^® system provides more accurate results without the pitfalls of colorimetric assays. By measuring amide bonds in protein chains, the system accurately quantitates an intrinsic component of every protein without relying on amino acid composition, dye binding properties or reduction-oxidation (redox) potential. You can evaluate major components of complex mixtures separable from the Amide I & II region, like lipids and carbohydrates—making lysates and membrane preps easier than ever!

Features & Benefits

• IR spectrometry measures amide bond absorbance for more reproducible quantitation across all proteins and peptides
  
• Improves accuracy over traditional colorimetric assays
  
• More compatible with detergents, reducing agents and other buffer components that interfere with traditional protein quantitation assays, providing more information from your sample
  
• Preserves precious samples—requires only 2 μL per analysis
  
• New software module can now quantify long aliphatic chains in lipids and detergents

Applications

Protein Quantitation, Lipid Analysis, Peptide Quantitation

Product Performance