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233153 Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dithiothreitol, DTT

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233153
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

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      233153-5GM
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          Description
          OverviewA protective agent for SH groups.
          Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
          Catalogue Number233153
          Brand Family Calbiochem®
          SynonymsDithiothreitol, DTT
          References
          ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
          Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
          Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
          Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
          Shaked, Z., et al. 1980. Biochemistry 19, 4156.
          Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          Product Information
          CAS number3483-12-3
          FormWhite solid
          Hill FormulaC₄H₁₀O₂S₂
          Chemical formulaC₄H₁₀O₂S₂
          Quality LevelMQ100
          Applications
          Biological Information
          Purity≥99% by titration
          Physicochemical Information
          ContaminantsHeavy metals: <1 ppm
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSEK1610000
          Safety Information
          R PhraseR: 22-36/38

          Harmful if swallowed.
          Irritating to eyes and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          233153-1GM 04055977217643
          233153-5GM 07790788054267

          Documentation

          Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem Certificates of Analysis

          TitleLot Number
          233153

          References

          Reference overview
          Chau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
          Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
          Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
          Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
          Shaked, Z., et al. 1980. Biochemistry 19, 4156.
          Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-August-2018 JSW
          SynonymsDithiothreitol, DTT
          DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.
          FormWhite solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number3483-12-3
          RTECSEK1610000
          Chemical formulaC₄H₁₀O₂S₂
          Purity≥99% by titration
          ContaminantsHeavy metals: <1 ppm
          SolubilityEthanol or H₂O (200 mM)
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Harmful
          Merck USA index14, 3376
          ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
          Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
          Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
          Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
          Shaked, Z., et al. 1980. Biochemistry 19, 4156.
          Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
          Cleland, W.W. 1964. Biochemistry 3, 480.