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194933 AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem

General proteolytic substrate.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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194933
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194933-5GM
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      Description
      OverviewGeneral proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.3. Mesh: >100.
      Catalogue Number194933
      Brand Family Calbiochem®
      References
      ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
      Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
      Product Information
      CAS number53092-90-3
      ATP CompetitiveN
      FormDark red solid
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetGeneral proteolytic substrate
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      194933-5GM 07790788048266

      Documentation

      AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      194933

      References

      Reference overview
      Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
      Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-October-2016 JSW
      DescriptionGeneral proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 5 mg/ml buffer, 10 min at 37°C, pH 7.0): <0.3. Mesh: >100.
      FormDark red solid
      Recommended reaction conditions
      Assay Protocol for Protease Activity 1. Dissolve 50 mg AZOCOLL® in 5 ml of buffer solution (e.g., PBS, pH 7.0) containing the proteolytic enzyme. 2. Incubate in stoppered tubes for 15 min at 37°C, agitating gently at 5 min intervals. 3. Cool the tubes in ice water and filter the contents through Whatman No. 1 filter paper. 4. Measure the absorbance at 520 nm, subtracting the blank value. 5. Plot absorbance versus protease activity. A linear relationship exists between protease concentration and the rate of dye liberation. Note: The rate of color yield from AZOCOLL® will vary with the individual proteases tested. This protocol is meant to serve as a guideline and may need to be modified for specific applications.
      CAS number53092-90-3
      SolubilityPBS (10 mg/ml)
      Storage Protect from light
      +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
      Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.