119101
Anti-H+,K+-ATPase, α-Subunit (1019-1034) Rabbit pAb
liquid, Calbiochem®
Product Name
Anti-H+,K+-ATPase, α-Subunit (1019-1034) Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, canine, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
Application
ELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Paraffin Sections (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Paraffin Sections (see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
This product has been discontinued.
Recognizes the α-subunit of H+,K+-ATPase.
Anti-H+,K+-ATPase, α-Subunit (1019-1034), rabbit polyclonal, recognizes the α-subunit of H+,K+-ATPase. It is validated for use in ELISA, WB, ICC, and IHC (paraffin sections).
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-subunit of H+,K+-ATPase.
Immunogen
Porcine
a synthetic peptide (GVRCCPGSWWDQGLYY) corresponding to amino acids 1019-1034 of porcine H+, K+-ATPase α-subunit
Other Notes
Exhibits some cross-reactivity with Na+, K+-ATPase at working dilution for H+,K+-ATPase. This antibody is also reported to work for ELISA, immunocytochemistry, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Smolka, A. 1992. Biophys. Biochem. Acta 1108, 75.
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C). Recommended dilution buffer is Tris buffered saline with 1% BSA, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ivana Novak et al.
The Journal of biological chemistry, 286(1), 280-289 (2010-10-28)
Pancreas secretes fluid rich in digestive enzymes and bicarbonate. The alkaline secretion is important in buffering of acid chyme entering duodenum and for activation of enzymes. This secretion is formed in pancreatic ducts, and studies to date show that plasma
Pawel J Schweiger et al.
Scientific reports, 8(1), 15255-15255 (2018-10-17)
The processes involved in renewal of the epithelium that lines the mouse stomach remain unclear. Apart from the cells in the isthmus, several other populations located deeper in the gastric glands have been suggested to contribute to the maintenance of
Ping He et al.
Molecular medicine reports, 11(3), 1911-1916 (2014-11-19)
Curcumin is known to possess anti‑inflammatory properties. Despite the fact that curcumin is known to be a strong inhibitor of H+, K+‑ATPase activity, the mechanism underlying the curcumin‑induced inhibition of the transcription of the H+, K+‑ATPase α subunit in gastric
S M C Przemeck et al.
American journal of physiology. Gastrointestinal and liver physiology, 292(2), G620-G627 (2006-10-28)
Unlike the small intestine and colon where gamma-radiation-induced apoptosis has previously been well characterized, the response of murine gastric epithelium to gamma-radiation has not been investigated in detail. Apoptosis was therefore assessed on a cell positional basis in gastric antral
Marco Tozzi et al.
Cancers, 12(3) (2020-03-14)
Pancreatic duct cells are equipped with acid/base transporters important for exocrine secretion. Pancreatic ductal adenocarcinoma (PDAC) cells may utilize such transporters to acidify extracellular tumor microenvironment, creating a niche favoring cell proliferation, fibrosis and resistance to chemotherapy-all contributing to the
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