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598226 Thiostrepton - CAS 1393-48-2 - Calbiochem

Thiostrepton, CAS 1393-48-2, is an antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit.

Thiostrepton - CAS 1393-48-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FOXM1 Inhibitor I

Primary Target: EF-G
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598226
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
1393-48-2C₇₂H₈₅N₁₉O₁₈S₅

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      598226-1GM
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          Description
          OverviewA thiazole-containing peptide antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit. Inhibits the function of elongation factor G (EF-G) and the dissociation of EF-G from the ribosome. The thiostrepton-resistant gene is also commonly used as a selective marker for recombinant DNA/plasmid technologies.
          Catalogue Number598226
          Brand Family Calbiochem®
          SynonymsFOXM1 Inhibitor I
          References
          ReferencesRodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
          Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
          Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
          Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
          Blanco, G., et al. 1992. Gene 112, 59.
          Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
          Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
          Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
          Smokvina, T., et al. 1990. Gene 94, 53.
          Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
          Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
          Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.
          Product Information
          CAS number1393-48-2
          FormOff-white powder
          Hill FormulaC₇₂H₈₅N₁₉O₁₈S₅
          Chemical formulaC₇₂H₈₅N₁₉O₁₈S₅
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetEF-G
          Purity≥98% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXN6300100
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          598226-10GM 04055977189162
          598226-1GM 07790788051914

          Documentation

          Thiostrepton - CAS 1393-48-2 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Thiostrepton - CAS 1393-48-2 - Calbiochem Certificates of Analysis

          TitleLot Number
          598226

          References

          Reference overview
          Rodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
          Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
          Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
          Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
          Blanco, G., et al. 1992. Gene 112, 59.
          Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
          Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
          Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
          Smokvina, T., et al. 1990. Gene 94, 53.
          Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
          Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
          Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.

          Citations

          Title
        • Sarae L. Bausch, Ekaterina Poliakova and David E. Draper. (2005) Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. Journal of Biological Chemistry 280, 29956-29963.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-August-2014 JSW
          SynonymsFOXM1 Inhibitor I
          DescriptionA thiazole-containing polypeptide antibiotic isolated from Streptomyces azureus. Inhibits bacterial protein synthesis and ribosomal GTPase activity by binding noncovalently, but virtually irreversibly, to the 23S rRNA in the GTPase center of the 50S subunit. Thiostrepton binding directly prevents binding to the ribosome of elongation factor G (EF-G), which also binds specifically to the 23S rRNA. Thiostrepton has proved useful in elucidating the molecular mechanisms of translation, particularly of ribosomal translocation. It is also used for selection, in bacteria of Streptomyces sp., of vectors containing the tsr gene (S. azureus), which encode a 23S rRNA methylase whose action prevents thiostrepton from binding to ribosomes.
          FormOff-white powder
          CAS number1393-48-2
          RTECSXN6300100
          Chemical formulaC₇₂H₈₅N₁₉O₁₈S₅
          Purity≥98% by HPLC
          SolubilityDMSO (10 mg/ml) or Chloroform (10 mg/ml)
          Storage Protect from moisture
          Protect from light
          +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          Merck USA index14, 9364
          ReferencesRodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
          Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
          Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
          Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
          Blanco, G., et al. 1992. Gene 112, 59.
          Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
          Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
          Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
          Smokvina, T., et al. 1990. Gene 94, 53.
          Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
          Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
          Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.
          Citation
        • Sarae L. Bausch, Ekaterina Poliakova and David E. Draper. (2005) Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. Journal of Biological Chemistry 280, 29956-29963.
          		•