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03-0203-00

SMC® Human GFAP High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human GFAP in human serum, plasma, and cerebrospinal fluid (CSF)

Synonym(s):

GFAP Single Molecule Counting Kit, GFAP ultra-sensitive immunoassay, Glial fibrillary acidic protein immunoassay kit

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About This Item

NACRES:
NA.77
UNSPSC Code:
12161503
Species reactivity:
human
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
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Product Name

SMC® Human GFAP High Sensitivity Immunoassay Kit,

Quality Segment

200

description

Used for ultrasensitive quantification of Human GFAP in human serum, plasma, and cerebrospinal fluid (CSF)

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

input

serum
cerebrospinal fluid (CSF)
plasma (K2 EDTA)

UniProt accession no.

P14136

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Glial fibrillary acidic protein (GFAP) is a key intermediate filament protein predominantly expressed in astrocytes, a type of glial cell in the central nervous system. GFAP plays a vital role in maintaining the structural integrity of astrocytes and supporting their functions, including the regulation of the extracellular environment, the provision of metabolic support to neurons, and the modulation of synaptic activity.

GFAP serves as an important biomarker for astrocyte activation and neuroinflammation. Elevated levels of GFAP in cerebrospinal fluid (CSF) and blood may indicate reactive astrogliosis, a condition often associated with brain injury, multiple sclerosis, Alzheimer′s disease, and other neurodegenerative disorders. As increased GFAP levels often reflect the extent of neuroinflammatory responses and neuronal damage, GFAP is a critical target to monitor when developing strategies aimed at mitigating neuroinflammation and preserving neuronal health.

Application

Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience

Features and Benefits

  • Femtogram-Level Aβ1-42 Detection: Achieves exceptional sensitivity (fg/mL) in serum, plasma or CSF.
  • Enhanced Signal Performance: Features a broadened dynamic range with reduced background noise, ensuring consistent results.
  • Familiar Assay Format: Utilizes a standard sandwich immunoassay design merged with advanced sensitivity.
  • Flexible Automation Integration: Incorporates with liquid handling systems to standardize workflows and further minimize variability.
  • Specialized Technical Support: Supported by SMC® platform experts.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 230 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 60-300,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 101% Plasma: 99% CSF: 101%
  • Recovery: Serum: 118% Plasma: 115% CSF: 101%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although GFAP was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Legal Information

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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