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524744 SIRT2, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sirtuin 2, His•Tag®, Human, Recombinant, E. coli

524744
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, human SIRT2 (amino acids 50-356) fused at the C-terminus to a His•Tag® sequence and expressed in E. coli. SIRT2 is a member of the class III histone deacetylase family and its activity is dependent on the co-factor, NAD+, which is cleaved during the deacetylation reaction. Sirtuins also possess an ADP-ribosyltransferase activity, that is unique to the class III HDACs. The active deacetylase is useful in the study of SIRT2 regulation and inhibitor screening.
      Catalogue Number524744
      Brand Family Calbiochem®
      SynonymsSirtuin 2, His•Tag®, Human, Recombinant, E. coli
      References
      ReferencesGrubisha, O., 2006. J.Biol.Chem. 281, 14057.
      Vaquero, A., 2006. Genes Dev. 20, 1256.8, 621.
      Frye, R.A., et al. 1999. Biochem. Biophys. Res. Commun. 260, 273.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enyme that will deacetylate 1 picomol fluorescent peptide substrate, Arg-His-Lys-Lys(Ac), per min at 30°C.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 0.05% Tween®-20, 50% glycerol, pH 8.0.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥1.4 U/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      524744 0

      Documentation

      SIRT2, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      524744

      References

      Reference overview
      Grubisha, O., 2006. J.Biol.Chem. 281, 14057.
      Vaquero, A., 2006. Genes Dev. 20, 1256.8, 621.
      Frye, R.A., et al. 1999. Biochem. Biophys. Res. Commun. 260, 273.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-April-08 JSW
      SynonymsSirtuin 2, His•Tag®, Human, Recombinant, E. coli
      DescriptionRecombinant, human SIRT2 (amino acids 50-356) fused at the C-terminus to a His•Tag® sequence and expressed in E. coli. SIRT2 is a member of the class III histone deacetylase family and its activity is dependent on the co-factor, NAD+, which is cleaved during the deacetylation reaction. Sirtuins also possess an ADP-ribosyltransferase activity, that is unique to the class III HDACs. The active deacetylase is useful in the study of SIRT2 regulation and inhibitor screening.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 0.05% Tween®-20, 50% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, 500 µM NAD+, 20 µM substrate [rg-His-Lys-Lys(Ac)], 100 ng/µl SIRT2, 37°C
      Purity≥90% by SDS-PAGE
      Specific activity≥1.4 U/µg protein
      Unit definitionOne unit is defined as the amount of enyme that will deacetylate 1 picomol fluorescent peptide substrate, Arg-His-Lys-Lys(Ac), per min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesGrubisha, O., 2006. J.Biol.Chem. 281, 14057.
      Vaquero, A., 2006. Genes Dev. 20, 1256.8, 621.
      Frye, R.A., et al. 1999. Biochem. Biophys. Res. Commun. 260, 273.