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HTS044RTA Ready-to-Assay™ M1 Acetylcholine (Muscarinic) Family Receptor Frozen Cells

Human M1 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_000738
HTS044RTA
1 kit  
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      Overview

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1M1
      Description
      Catalogue NumberHTS044RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ M1 Acetylcholine (Muscarinic) Family Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M1 receptor, along with the M3 and M5 receptors, signal through Gq/11 and subsequent release of Ca++ from the ER. The M1 receptor is expressed in ganglia and mediates depolarization of ganglia by inhibition of voltage-gated M-type K+ channels. In addition, the M1 receptor mediates venous contraction (Caulfield and Birdsall, 1998). Millipore’s cloned human M1 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant M1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at M1.
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationHuman M1 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • CHRM1
      GPCR ClassA
      Host CellsChem-1
      Protein TargetM1
      Target Sub-FamilyAcetylcholine (muscarinic)
      Cell Line Type
      • GPCR Cell Lines
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size1 kit
      Global Trade Item Number
      Catalogue Number GTIN
      HTS044RTA 04053252332593