Millipore Sigma Vibrant Logo

537059 Propidium Iodide - CAS 25535-16-4 - Calbiochem

Membrane impermeable DNA intercalator.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
537059
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
25535-16-4C₂₇H₃₄N₄I₂

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
537059-100MG
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 100 mg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      537059-1GM
      Retrieving availability...
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic ampoule 1 gm
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          537059-250MG
          Retrieving availability...
          —
          Limited Availability Limited Availability
          In Stock 
          Discontinued
          Limited Quantities Available
          Availability to be confirmed
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              		 Plastic ampoule 250 mg
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity is a multiple of
              Maximum Quantity is
              Upon Order Completion More Information
              You Saved ()
               
              —
              Request Pricing
              		 —
              537059-50MG
              Retrieving availability...
              —
              Limited Availability Limited Availability
              In Stock 
              Discontinued
              Limited Quantities Available
              Availability to be confirmed
                Remaining : Will advise
                  Remaining : Will advise
                  Will advise
                  Contact Customer Service
                  Contact Customer Service

                  		 Glass bottle 50 mg
                  Retrieving price...
                  Price could not be retrieved
                  Minimum Quantity is a multiple of
                  Maximum Quantity is
                  Upon Order Completion More Information
                  You Saved ()
                   
                  —
                  Request Pricing
                  		 —
                  Description
                  OverviewMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
                  Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
                  Catalogue Number537059
                  Brand Family Calbiochem®
                  References
                  ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
                  Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
                  Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
                  de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
                  Pollice, A.A., et al. 1992. Cytometry 13, 432.
                  Product Information
                  CAS number25535-16-4
                  ATP CompetitiveN
                  FormDark red solid
                  Hill FormulaC₂₇H₃₄N₄I₂
                  Chemical formulaC₂₇H₃₄N₄I₂
                  ReversibleN
                  Structure formula ImageStructure formula Image
                  Quality LevelMQ100
                  Applications
                  Biological Information
                  Primary TargetMembrane impermeable DNA intercalator
                  Sample TypeIntact Cells
                  Physicochemical Information
                  Cell permeableN
                  Emission max.
                  Excitation max.
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  RTECSSF7949600
                  Safety Information
                  R PhraseR: 36/37/38

                  Irritating to eyes, respiratory system and skin.
                  S PhraseS: 26

                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Irritant
                  Storage +2°C to +8°C
                  Protect from Light Protect from light
                  Do not freeze Ok to freeze
                  Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Catalogue Number GTIN
                  537059-100MG 04055977195897
                  537059-1GM 04055977195880
                  537059-250MG 04055977195903
                  537059-50MG 04055977195910

                  Documentation

                  Propidium Iodide - CAS 25535-16-4 - Calbiochem SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Propidium Iodide - CAS 25535-16-4 - Calbiochem Certificates of Analysis

                  TitleLot Number
                  537059

                  References

                  Reference overview
                  Belloc, F., et al. 1994. Cytometry 17, 59.
                  Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
                  Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
                  de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
                  Pollice, A.A., et al. 1992. Cytometry 13, 432.

                  Citations

                  Title
                • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
                • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
                  		•
                  Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision29-August-2008 RFH
                  DescriptionMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
                  FormDark red solid
                  CAS number25535-16-4
                  RTECSSF7949600
                  Chemical formulaC₂₇H₃₄N₄I₂
                  Structure formulaStructure formula
                  SolubilityH₂O. Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
                  Storage Protect from light
                  +2°C to +8°C
                  Do Not Freeze Ok to freeze
                  Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
                  Toxicity Irritant
                  ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
                  Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
                  Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
                  de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
                  Pollice, A.A., et al. 1992. Cytometry 13, 432.
                  Citation
                • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
                • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
                  		•