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CC130 PluriSTEM-XF™ Recombinant Vitronectin

Human pluripotent stem cell culture.

PluriSTEM-XF™ Recombinant Vitronectin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Attachment, Cell Culture, Stem Cell Culture
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CC130
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cell Attachment, CULT, Cult
      Description
      Catalogue NumberCC130
      Trade Name
      • PluriSTEM
      DescriptionPluriSTEM-XF™ Recombinant Vitronectin
      Alternate Names
      • S-protein
      • Serum-spreading factor
      • V75
      Background InformationPluriSTEM™ Recombinant Vitronectin is an effective xeno-free alternative to matrigel™ and has been validated to successully culture human ES and iPS cells using PluriSTEM™ Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

      PluriSTEM™ Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
      References
      Product Information
      HS Code3821 00 00
      PresentationProduct is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.
      Quality LevelMQ100
      Applications
      ApplicationHuman pluripotent stem cell culture.
      Key Applications
      • Cell Attachment
      • Cell Culture
      • Stem Cell Culture
      Application NotesSupports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
      Biological Information
      Concentration0.5 mg/mL
      HostE. coli
      PurityProduct is ≥ 92% pure by SDS-PAGE.
      Gene Symbol
      • VTN
      UniProt Number
      Stem Cell Type
      • Human Embryonic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC130 04053252938511