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PC461 PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-6) Rabbit pAb

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PC461
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~53 kDa p53 protein phosphorylated at Ser15. Does not recognizes unphosphorylated p53.
      Catalogue NumberPC461
      Brand Family Calbiochem®
      Application Data
      Detection of p53, phosphorylated at Ser15 by staining paraffin sections. Sample: Human prostate carcinoma. Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb (Cat. No. PC461) (1:1000). Detection: DAB.
      References
      ReferencesLevine, A.J. 1997. Cell 88, 323.
      Meek, D.W. 1997. Pathol. Biol. Paris 45, 804.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Cho, Y., et al. 1994. Science 265, 346.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlMCF7 cells or prostate carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1:5000-1:10,000)
      Paraffin Sections (1:1000-1:3000, heat pre-treatment required)
      Application CommentsDoes not recognizes unphosphorylated p53. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide [VEPPLpSQETFS(C)] corresponding to amino acids 10-20 surrounding the Ser¹⁵ phosphorylation site of human p53
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC461 0

      Documentation

      PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-6) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-6) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC461

      References

      Reference overview
      Levine, A.J. 1997. Cell 88, 323.
      Meek, D.W. 1997. Pathol. Biol. Paris 45, 804.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Cho, Y., et al. 1994. Science 265, 346.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2007 RFH
      ApplicationImmunoblotting (1:5000-1:10,000)
      Paraffin Sections (1:1000-1:3000, heat pre-treatment required)
      Application Data
      Detection of p53, phosphorylated at Ser15 by staining paraffin sections. Sample: Human prostate carcinoma. Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb (Cat. No. PC461) (1:1000). Detection: DAB.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~53 kDa p53 phoshorylated at Ser15.
      Backgroundp53 (393 amino acids) exhibits sequence-specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage by inducing GADD45, WAF1, and MDM2. p53 is the most commonly mutated gene in human cancer with most mutations occurring in the DNA contact residues. p53 binds damaged DNA non-specifically via its C-terminal 75 amino acids which in turn may be responsible for subsequent activation, sequence-specific DNA binding, and WAF1 induction allowing DNA repair to take place. p53 down regulates bcl-2 expression and up regulates bax expression, but may not always be necessary for apoptosis. In addition to the afore mentioned proteins, two other proteins involved in replication and recombination, RPA and RAD51, also bind p53. Multi-site phosphorylation of p53 is key in mediating many of these events. Within its N-terminal domain, p53 is phosphorylated by kinases responsive to UV radiation, cytokines, DNA damage and growth factors. C-terminal phosphorylation is mediated by protein kinases involved in growth stimulation, cell cycle control and apoptosis. These C-terminal phosphorylation events are involved in controlling the specific DNA binding function of p53, and may play a role in regulating other functions of p53.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide [VEPPLpSQETFS(C)] corresponding to amino acids 10-20 surrounding the Ser¹⁵ phosphorylation site of human p53
      IsotypeIgG
      Specieshuman
      Positive controlMCF7 cells or prostate carcinoma tissue
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsDoes not recognizes unphosphorylated p53. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLevine, A.J. 1997. Cell 88, 323.
      Meek, D.W. 1997. Pathol. Biol. Paris 45, 804.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Cho, Y., et al. 1994. Science 265, 346.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.

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