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PC386 PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

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PC386-50UL
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      Description
      OverviewRecognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
      Catalogue NumberPC386
      Brand Family Calbiochem®
      Application Data
      Detection of human p53, phophorylated on Ser15, by immunoblotting. Samples: Whole cell lysates from untreated HCT116 cells (Lane A) and HCT116 cells treated with ionizing radiation (Lane B). Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb (Cat. No. PC386) (1:1000). Detection: chemiluminescence.
      References
      ReferencesTibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
      Levine, A.J. 1997. Cell 88, 323.
      Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
      Shieh, S.Y., et al. 1997. Cell 91, 325.
      Meek, D.W. 1994. Semin Cancer Biol. 5, 203.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlPC-12, HCT116, or HeLa cells treated with DNA damaging agents
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (1:200)
      Application CommentsDoes not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Global Trade Item Number
      Catalogue Number GTIN
      PC386-50UL 04055977224559