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106888
Sigma-AldrichPapanicolaou's solution 2a Orange G solution (OG 6)
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Description
Catalogue Number
106888
Description
Papanicolaou's solution 2a Orange G solution (OG 6)
Overview
Papanicolaou’s solution 2a Orange G solution (OG6) - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, cervical smears. Papanicolaou’s technique is the most used staining procedure for cytological specimens and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution, (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Papanicolaou’s solution 2a Orange G solution (OG6) gives a pale, yellow-orange staining result with mature and keratinized squamous cells.
The 500 ml bottle provides 1500 - 2000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233: Keep container tightly closed. P240: Ground and bond container and receiving equipment. P241: Use explosion-proof electrical/ ventilating/ lighting equipment. P242: Use non-sparking tools. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal Word
Danger
Storage class
3 Flammable liquids
WGK
WGK 2 obviously hazardous to water
Disposal
28 Aqueous solutions: Container D.
Safety Information
Categories of danger
highly flammable
Product Usage Statements
Storage and Shipping Information
Storage
Store at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RID
UN 1170 , 3, II
Declaration (transport by air) IATA-DGR
UN 1170 , 3, II
Declaration (transport by sea) IMDG-Code
UN 1170 , 3, II
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)
passes test
Nuclei
blue to dark violet
Cyanophilic Cytoplasm (basophilic)
blue-green
Eosinophilic Cytoplasm (acidophilic)
pink
Global Trade Item Number
Catalogue Number
GTIN
1068880500
04022536084145
1068881000
04022536676579
1068882500
04022536084169
Documentation
Papanicolaou's solution 2a Orange G solution (OG 6) SDS
