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482251 NIPP-1, His•Tag®, Bovine Thymus, Recombinant, E. coli - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Nuclear Inhibitor of Protein Phosphatase-1

Primary Target: PP1
482251
  
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      Overview

      Replacement Information
      Description
      OverviewRecombinant, bovine NIPP-1 fused to a His•Tag® sequence and expressed in E. coli. Potent and specific inhibitor of protein phosphatase 1 (PP1; Ki = 1-10 pM) that can be used to distinguish PP1 from other major serine/threonine protein phosphatases including PP2A, PP2B, and PP2C. Also an ideal substrate for protein kinase A and casein kinase II. Thought to be involved in the targeting of PP1 to RNA-associated substrates and may also be involved in the dephosphorylation of transcription factors like CREB and the tumor suppressor Rb.

      This product has been discontinued.
      Catalogue Number482251
      Brand Family Calbiochem®
      SynonymsNuclear Inhibitor of Protein Phosphatase-1
      References
      ReferencesBeullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
      Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
      Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
      Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
      Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
      Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
      Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.
      Product Information
      ATP CompetitiveN
      FormLiquid
      FormulationIn 500 mM NaCl, 20 mM Tris, 500 µM benzamidine, 500 µM PMSF, 5 µM leupeptin, pH 7.5.
      ReversibleN
      Applications
      Biological Information
      Primary TargetPP1
      Primary Target K<sub>i</sub>1-10 pM against protein phosphatase 1 (PP1)
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      482251 0

      Documentation

      NIPP-1, His•Tag®, Bovine Thymus, Recombinant, E. coli - Calbiochem Certificates of Analysis

      TitleLot Number
      482251

      References

      Reference overview
      Beullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
      Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
      Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
      Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
      Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
      Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
      Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-November-2009 RFH
      SynonymsNuclear Inhibitor of Protein Phosphatase-1
      DescriptionRecombinant, bovine NIPP-1 fused to a His•Tag® sequence and expressed in E. coli. Potent and specific inhibitor or protein phosphatase 1 (PP1; Ki = 1-10 pM) that can be used to distinguish PP1 from other major serine/threonine protein phosphatases, including PP2A, PP2B, and PP2C. Reported to be an ideal substrate for protein kinase A (PKA) and casein kinase II (CKII). Believed to be involved in the targeting of PP1 to RNA-associated substrates. Also thought to be involved in the dephosphorylation of transcription factors like CREB and the tumor suppressor protein, Rb.
      FormLiquid
      FormulationIn 500 mM NaCl, 20 mM Tris, 500 µM benzamidine, 500 µM PMSF, 5 µM leupeptin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBeullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
      Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
      Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
      Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
      Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
      Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
      Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.