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14-691M MRCKα Protein, active, 250 µg

Active, C-terminal 6His-tagged, recombinant, human MRCKα, amino acids 1-473. For use in Kinase Assays.

MRCKα Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-691M
250 µg  Also available in 10 μg size (cat#14-691) and in bulk (cat# 14-691-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-691M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMRCKα Protein, active, 250 µg
      OverviewC-terminal 6His-tagged, recombinant, human MRCKα, amino acids 1-473.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, C-terminal 6His-tagged, recombinant, human MRCKα, amino acids 1-473. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceMRCKα, amino acids 1-473, expressed by baculovirus in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.
      Gene Symbol
      • CDC42BPA
      • DKFZp686L1738
      • PK428
      • DKFZp686P1738
      • KIAA0451
      • FLJ23347
      • MRCK
      • MRCKA
      Protein TargetMRCKα
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q5VT25 # May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
      COFACTOR: Magnesium.
      SIZE: 1732 amino acids; 197307 Da
      SUBUNIT: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity).
      TISSUE SPECIFICITY: Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
      SIMILARITY: SwissProt: Q5VT25 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 CNH domain. & Contains 1 CRIB domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
      Molecular Weight55.3kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of S6 Kinase/Rsk2 Substrate Peptide 2 (KKRNRTLTV)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C from date of shipment
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-691) and in bulk (cat# 14-691-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-691M 04053252580932

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-691 MRCKα Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases