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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

RTHYMAG-30K MilliporeMILLIPLEX MAP Rat Thyroid Magnetic Bead Panel - Endocrine Multiplex Assay

The analytes available for this multiplex kit are: TSH, T3, T4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
References
Technical Information

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Endocrine

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RTHYMAG-30K

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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

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Overview

Replacement Information

Key Spec Table

Analytes Available	Species Reactivity	Key Applications	Detection Methods
TSH T3 T4	R	Mplex	Luminex xMAP

Description
Catalogue Number	RTHYMAG-30K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Rat Thyroid Magnetic Bead Panel - Endocrine Multiplex Assay
Background Information	Triiodothyronine (T3) and thyroxine (T4) are key hormones secreted from the thyroid gland with a wide-range of biological effects including the metabolism of carbohydrates, fatty acids, and proteins. In addition these hormones play a vital role in the development of the central nervous and skeletal systems. The syntheses and release of T3 and T4 are regulated by Thyroid-Stimulating Hormone (TSH), which is secreted from the anterior pituitary gland. Together, these thyroid hormones play critical roles in the regulation of energy balance and mental and physical development. According to some studies, the simultaneous measurement of T3, T4 and TSH is of greater value than individual measurements, especially when monitoring disease relapse or therapeutic response.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Intra-assay: < 10% Inter-assay: < 15%
Panel Type	MAGNETIC Endocrine
Quality Level	MQ200

Applications
Application	The analytes available for this multiplex kit are: TSH, T3, T4.
Key Applications	Multiplexing
Application Notes	This assay is an overnight assay (4ºC) requiring 25 μL of diluted (1:5 recommended) serum, plasma or tissue culture supernatants

Biological Information
Species Reactivity	Rat
Analytes Available	TSH T3 T4
Cross Reactivity	There was no or negligible cross-reactivity between the analytes within the panel.
Accuracy	TSH 89% T3 105% T4 131%

Physicochemical Information
Sensitivity	TSH:38 pg/mL T3: 284 pg/mL T4: 2255 pg/mL
Linearity of Dilution	Contact technical support for this information.
Standard Curve Range	TSH & T3: 41-10,000 pg/mL T4: 823-200,00 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information

Packaging Information
Material Size	96-well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
RTHYMAG-30K	04053252988622

Documentation

MILLIPLEX MAP Rat Thyroid Magnetic Bead Panel - Endocrine Multiplex Assay SDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
The prospective protective effect of selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid. Hassanin, KM; Abd El-Kawi, SH; Hashem, KS International journal of nanomedicine 8 1713-20 2013 Show Abstract Nanotechnology has enabled researchers to synthesize nanosize particles that possess increased surface areas. Compared to conventional microparticles, it has resulted in increased interactions with biological targets.The objective of this study was to determine the protective ability of selenium nanoparticles against hexavalent chromium-induced thyrotoxicity.Twenty male rats were used in the study, and arbitrarily assigned to four groups. Group 1 was the control group, and was given phosphate-buffered saline. Group 2 was the chromium-treated group and was given K2Cr2O7 60 μg/kg body weight intraperitoneally as a single dose on the third day of administration. Group 3 was the nano-selenium-treated group and was given selenium nanoparticles (size 3-20 nm) 0.5 mg/kg body weight intraperitoneally daily for 5 consecutive days. Group 4 was the nano-selenium chromium-treated group, which received selenium nanoparticles for 5 days and a single dose of K2Cr2O7 on the third day of administration.Blood samples were collected from rats for measuring thyroid hormones (free triiodothyronine [T3] and free thyroxine [T4]) and oxidative and antioxidant parameters (malondialdehyde [MDA], reduced glutathione [GSH], catalase, and superoxide dismutase [SOD]). Upon dissection, thyroid glands were taken for histopathological examination by using paraffin preparations stained with hematoxylin and eosin (H&E) and Masson's trichrome. Immunohistochemical staining was performed for detecting cellular proliferation using Ki67 antibodies.The present study shows that K2Cr2O7 has a toxic effect on the thyroid gland as a result of inducing a marked oxidative damage and release of reactive oxygen species. This was shown by the significant decrease in free T3 and T4 and GSH levels, which was accompanied by significant increases in catalase, SOD, and MDA in the chromium-treated group compared to the control group. Se nanoparticles have a protective effect on K2Cr2O7-induced thyroid damage, as a result of correcting the free T3 and T4 levels and GSH, catalase, SOD, and MDA compared to the K2Cr2O7-treated group. Administration of nano-selenium alone in the nano-selenium-treated group had no toxic effect on rats' thyroid compared to the control group. The biochemical results were confirmed by histopathological, immunohistochemical and pathomorphological studies.	23658489

Reference overview

Pub Med ID

The prospective protective effect of selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid.
Hassanin, KM; Abd El-Kawi, SH; Hashem, KS
International journal of nanomedicine 8 1713-20 2013

Show Abstract

Nanotechnology has enabled researchers to synthesize nanosize particles that possess increased surface areas. Compared to conventional microparticles, it has resulted in increased interactions with biological targets.The objective of this study was to determine the protective ability of selenium nanoparticles against hexavalent chromium-induced thyrotoxicity.Twenty male rats were used in the study, and arbitrarily assigned to four groups. Group 1 was the control group, and was given phosphate-buffered saline. Group 2 was the chromium-treated group and was given K2Cr2O7 60 μg/kg body weight intraperitoneally as a single dose on the third day of administration. Group 3 was the nano-selenium-treated group and was given selenium nanoparticles (size 3-20 nm) 0.5 mg/kg body weight intraperitoneally daily for 5 consecutive days. Group 4 was the nano-selenium chromium-treated group, which received selenium nanoparticles for 5 days and a single dose of K2Cr2O7 on the third day of administration.Blood samples were collected from rats for measuring thyroid hormones (free triiodothyronine [T3] and free thyroxine [T4]) and oxidative and antioxidant parameters (malondialdehyde [MDA], reduced glutathione [GSH], catalase, and superoxide dismutase [SOD]). Upon dissection, thyroid glands were taken for histopathological examination by using paraffin preparations stained with hematoxylin and eosin (H&E) and Masson's trichrome. Immunohistochemical staining was performed for detecting cellular proliferation using Ki67 antibodies.The present study shows that K2Cr2O7 has a toxic effect on the thyroid gland as a result of inducing a marked oxidative damage and release of reactive oxygen species. This was shown by the significant decrease in free T3 and T4 and GSH levels, which was accompanied by significant increases in catalase, SOD, and MDA in the chromium-treated group compared to the control group. Se nanoparticles have a protective effect on K2Cr2O7-induced thyroid damage, as a result of correcting the free T3 and T4 levels and GSH, catalase, SOD, and MDA compared to the K2Cr2O7-treated group. Administration of nano-selenium alone in the nano-selenium-treated group had no toxic effect on rats' thyroid compared to the control group. The biochemical results were confirmed by histopathological, immunohistochemical and pathomorphological studies.

23658489

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- RTH-119 and RTH-219
Quality Control Ranges - Milliplex Rat Thyroid Hormone and Milliplex Rat Thyroid Hormone Magnetic Bead Panel
RTHYMAG-30K xPonent Protocol Template Version Magpix
RTHYMAG-30K xPonent Standard Template Version Magpix

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Select A Panel Type	.
Select A Kit	.
Select A Sample Type	For some panels, sample type will determine which analytes can be plexed together.

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