Millipore Sigma Vibrant Logo

17-191 MAP Kinase/Erk Assay Kit, non-radioactive

View Products on Sigmaaldrich.com
17-191
1 kit  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsAnalytes Available
      KAMAP Kinase/Erk
      Description
      Catalogue Number17-191
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMAP Kinase/Erk Assay Kit, non-radioactive
      References
      Product Information
      Components
      • Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
      • MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
      • Inhibitor Cocktail (Cat.# 20-116)
      • Magnesium/ATP Cocktail (Cat.# 20-113)
      • Anti-phospho-MBP, clone P12 (Cat.# 05-429)
      Quality LevelMQ100
      Applications
      ApplicationMAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.
      Key Applications
      • Kinase Assay
      Biological Information
      Analytes Available
      • MAP Kinase/Erk
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      • ERK2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. The assay kit is based on phosphorylation of a specific substrate (myelin basic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody.
      Sales RestrictionsThis product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      17-191 04053252618536

      Documentation

      MAP Kinase/Erk Assay Kit, non-radioactive SDS

      Title

      Safety Data Sheet (SDS) 

      MAP Kinase/Erk Assay Kit, non-radioactive Certificates of Analysis

      TitleLot Number
      MAP Kinase Assay Kit 2430444
      MAP Kinase Assay Kit (Non-Radioactive) - 2135180 2135180
      MAP Kinase Assay Kit (Non-Radioactive) 3027320
      MAP Kinase Assay Kit (Non-Radioactive) 2910027
      MAP Kinase Assay Kit (Non-Radioactive) 3046574
      MAP Kinase Assay Kit (Non-Radioactive) 3150678
      MAP Kinase Assay Kit (Non-Radioactive) 2961359
      MAP Kinase Assay Kit (Non-Radioactive) - 2275608 2275608
      MAP Kinase Assay Kit (Non-Radioactive) - 3215720 3215720
      MAP Kinase Assay Kit (Non-Radioactive) - 3385040 3385040

      References

      Reference overviewPub Med ID
      Prion protein detection using nanomechanical resonator arrays and secondary mass labeling.
      Madhukar Varshney,Philip S Waggoner,Christine P Tan,Keith Aubin,Richard A Montagna,Harold G Craighead
      Analytical chemistry  80  2008

      Show Abstract
      18271602 18271602
      Capillary electrophoresis of pesticides: V. Analysis of pyrethroid insecticides via their hydrolysis products labeled with a fluorescing and UV absorbing tag for laser-induced fluorescence and UV detection.
      A Karcher,Z El Rassi
      Electrophoresis  18  1997

      Show Abstract
      9237575 9237575
      Involvement of lysine residues in the gating of the ryanodine receptor/Ca2+-release channel of skeletal muscle sarcoplasmic reticulum.
      W Feng,V Shoshan-Barmatz
      European journal of biochemistry / FEBS  247  1997

      Show Abstract
      9288920 9288920
      Characterization of the expression and gene promoter of CD22 in murine B cells.
      K B Andersson,K E Draves,D M Magaletti,S Fujioka,K L Holmes,C L Law,E A Clark
      European journal of immunology  26  1996

      Show Abstract
      8977319 8977319
      Characterization of cytokine-producing cells in mucosal effector sites: CD3+ T cells of Th1 and Th2 type in salivary gland-associated tissues.
      T Hiroi,K Fujihashi,J R McGhee,H Kiyono
      European journal of immunology  24  1994

      Show Abstract
      7957557 7957557
      Determination of human tumour necrosis factor-alpha (TNF-alpha) by time-resolved immunofluorometric assay.
      U Turpeinen,U H Stenman
      Scandinavian journal of clinical and laboratory investigation  54  1994

      Show Abstract
      7809581 7809581
      Molecular cloning and chemical synthesis of a novel antibacterial peptide derived from pig myeloid cells.
      M Zanetti,P Storici,A Tossi,M Scocchi,R Gennaro
      The Journal of biological chemistry  269  1994

      Show Abstract
      8132502 8132502
      Cell surface display of rat invariant gamma chain: detection by monoclonal antibodies directed against a C-terminal gamma chain segment.
      A Fisch,K Reske
      European journal of immunology  22  1992

      Show Abstract
      1601033 1601033
      Processing of the precursor of protamine P2 in mouse. Peptide mapping and N-terminal sequence analysis of intermediates.
      D Carré-Eusèbe,F Lederer,K H Lê,S M Elsevier
      The Biochemical journal  277 ( Pt 1)  1991

      Show Abstract Full Text Article
      1854346 1854346
      Langerhans cells and T lymphocyte subsets in the murine vagina and cervix.
      M B Parr,E L Parr
      Biology of reproduction  44  1991

      Show Abstract
      2015366 2015366

      Related Products & Applications

      Kit Component

      Catalogue Number Description  
      05-429 Anti-phospho-MBP Antibody, clone P12 Show Pricing & Availability
      20-108 Assay Dilution Buffer I (ADBI) Show Pricing & Availability
      20-113 Magnesium/ATP Cocktail Show Pricing & Availability
      20-116 Kinase Inhibitor Cocktail Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays