Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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407652
Sigma-AldrichInterleukin-6, Human, Recombinant, E. coli
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Description
Overview
Recombinant, human, interleukin-6 expressed in E. coli. A prototypic pleiotrophic, pro-inflammatory cytokine that stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is produced by macrophages, endothelial cells, and activated T helper cells. Also considered as a potential marker of adipocyte differentiation. Human IL-6 is active on murine cells.
Catalogue Number
407652
Brand Family
Calbiochem®
Synonyms
rhIL-6
References
References
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9. Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890. Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182. Liu, J., et al. 1994. Cell Immunol.155, 229. Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
Interleukin-6, Human, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
407652
References
Reference overview
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9. Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890. Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182. Liu, J., et al. 1994. Cell Immunol.155, 229. Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
26-October-2007 JSW
Synonyms
rhIL-6
Description
Recombinant, human, interleukin-6 expressed in E. coli. Interleukin-6 is a protein produced by a number of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity. Stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B-cells, T-cells, and hepatocytes. Stimulates growth and inhibits constitutive protein synthesis-independent apoptosis of murine B-cell hybridoma 7TD1. Human sequence IL-6 is active on murine cells. 1 µg is equivalent to 7.0 x 103 WHO units.
Form
Lyophilized
Formulation
Carrier free.
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg IL-6
Biological activity
8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.
Solubility
Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9. Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890. Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182. Liu, J., et al. 1994. Cell Immunol.155, 229. Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
