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400052 Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      31282-04-9C₂₀H₃₇N₃O₁₃
      Description
      OverviewSterile liquid formulation of Cat. No. 400050.
      Catalogue Number400052
      Brand Family Calbiochem®
      References
      ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
      Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
      Cullen, D., et al. 1987. Gene 57, 21.
      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
      Santerre, R.F., et al. 1984. Gene 30, 147.
      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
      Product Information
      CAS number31282-04-9
      FormLiquid
      Formulation50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
      Hill FormulaC₂₀H₃₇N₃O₁₃
      Chemical formulaC₂₀H₃₇N₃O₁₃
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.
      Biological Information
      Biological activityPotency: ≥1000 U/mg
      Purity≥85% by HPLC and TLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSWK2130000
      Safety Information
      R PhraseR: 26/27/28-37/38-41

      Very toxic by inhalation, in contact with skin and if swallowed.
      Irritating to respiratory system and skin.
      Risk of serious damage to eyes.
      S PhraseS: 23-26-36/37/39-45

      Do not breathe fumes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic & Carcinogenic / Teratogenic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      400052 0

      Documentation

      Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      400052

      References

      Reference overview
      Sugimoto, K., et al. 1994. Plant J. 5, 863.
      Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
      Cullen, D., et al. 1987. Gene 57, 21.
      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
      Santerre, R.F., et al. 1984. Gene 30, 147.
      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-October-2007 RFH
      DescriptionHygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
      FormLiquid
      Formulation50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.
      CAS number31282-04-9
      RTECSWK2130000
      Chemical formulaC₂₀H₃₇N₃O₁₃
      Structure formulaStructure formula
      Purity≥85% by HPLC and TLC
      Biological activityPotency: ≥1000 U/mg
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Highly Toxic & Carcinogenic / Teratogenic
      Merck USA index14, 4852
      ReferencesSugimoto, K., et al. 1994. Plant J. 5, 863.
      Lee, M.G., and van der Ploeg, L.H. 1991. Gene 105, 255.
      Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34, 1915.
      Cullen, D., et al. 1987. Gene 57, 21.
      Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
      Santerre, R.F., et al. 1984. Gene 30, 147.
      Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.

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      Categories

      Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents
      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents