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48-602MAG
Buffer Detection Kit for Magnetic Beads
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400087
Sigma-AldrichHIF-2α Translation Inhibitor - CAS 882268-69-1 - Calbiochem
The HIF-2α Translation Inhibitor, also referenced under CAS 882268-69-1, controls the biological activity of HIF-2α. This small molecule/inhibitor is primarily used for Cell Structure applications.
More>>The HIF-2α Translation Inhibitor, also referenced under CAS 882268-69-1, controls the biological activity of HIF-2α. This small molecule/inhibitor is primarily used for Cell Structure applications. Less<<
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Description
Overview
A cell-permeable thienylhydrazone compound that suppresses the translation of cellular HIF-2α message by enhancing the binding of IRP1 (Iron-Regulatory Protein 1) to the IRE (Iron-Responsive Element) region at the 5' UTR of the HIF-2α mRNA, exhibiting otherwise no effects toward the transcription of HIF-2α mRNA or the stability of HIF-2α mRNA and protein. Shown to reduce cellular protein levels of HIF-2α and HIF-2α target genes, including Glut-1, IGFBP3, and VEGF, in 786-O cells (5 to 10 µM final concentration) that express only the 2α isoform of hypoxia-inducible factor.
Catalogue Number
400087
Brand Family
Calbiochem®
Synonyms
Hypoxia-Inducible Factor-2α Translation Inhibitor, Methyl-3-(2-(cyano(methylsulfonyl)methylene)hydrazino)thiophene-2-carboxylate, HIF Inhibitor II
HIF-2α Translation Inhibitor - CAS 882268-69-1 - Calbiochem Certificates of Analysis
Title
Lot Number
400087
References
Reference overview
Zimmer, M., et al. 2008. Mol. Cell32, 838.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
12-April-2011 RFH
Synonyms
Hypoxia-Inducible Factor-2α Translation Inhibitor, Methyl-3-(2-(cyano(methylsulfonyl)methylene)hydrazino)thiophene-2-carboxylate, HIF Inhibitor II
Description
A cell-permeable thienylhydrazone compound that suppresses the translation of cellular HIF-2α message by enhancing the binding of IRP1 (Iron-Regulatory Protein 1) to the IRE (Iron-Responsive Element) region at the 5' UTR of the HIF-2α mRNA, exhibiting otherwise no effects toward the transcription of HIF-2α mRNA or the stability of HIF-2α mRNA and protein. Shown to reduce cellular protein levels of HIF-2α and HIF-2α target genes, including Glut-1, IGFBP3, and VEGF, in 786-O cells (5-10 µM final concentration) that express only the 2α isoform of hypoxia-inducible factor.
Form
Amber solid
Intert gas (Yes/No)
Packaged under inert gas
CAS number
882268-69-1
Chemical formula
C₉H₉N₃O₄S₂
Structure formula
Purity
≥95% (sum of two isomers)
Solubility
DMSO (5 mg/ml)
Storage
Protect from light +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.