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344289 Freund’s Complete Adjuvant, Modified, Mycobacterium sp.

Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum, CAS 9007-81-2, provided as a set of 10 vials each containing: 85% Drakeol 5NF, 15% Arlacel A, and 0.1% M.butyricum dry cells.

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344289
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      Description
      OverviewContains 10 vials per set, each containing 10 ml of Freund's complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium sp. dry cells.
      Catalogue Number344289
      Brand Family Calbiochem®
      Product Information
      CAS number9007-81-2
      FormClear amber to slightly cloudy liquid
      FormulationYellow, oily, bacterial suspension.
      Quality LevelMQ100
      Applications
      ApplicationFreund’s Complete Adjuvant, Modified, Mycobacterium butyricum, CAS 9007-81-2, provided as a set of 10 vials each containing: 85% Drakeol 5NF, 15% Arlacel A, and 0.1% M.butyricum dry cells.
      Application NotesImmunization
      Application CommentsComposition
      The Freund's Adjuvant set contains 10 identical ampules, each with 10 ml of Freund's Complete Adjuvant homogenized in the following preparations by weight:
      85% Drakeol 5 NF
      15% Arlacel A, mannide monooleate emulsifier
      0.1% Mycobacterium sp. dry cells

      Sample Preparation
      1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
      2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
      3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
      4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
      Safety Information according to GHS
      RTECSLS6860000
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      344289-1SET 04055977214697