Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
343242
Sigma-AldrichFLUO 3/AM - CAS 121714-22-5 - Calbiochem
Membrane-permeable version of FLUO 3. Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.
More>>Membrane-permeable version of FLUO 3. Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+. Less<<
FLUO 3/AM - CAS 121714-22-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
The quantity field is empty. Please enter a quantity of 1 or more to add items to your cart.
Description
Overview
Membrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.
Caputo, C., and Bolanos, P. 1994. J. Physiol. (London)481, 119. Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134. Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun.163, 309.
FLUO 3/AM - CAS 121714-22-5 - Calbiochem Certificates of Analysis
Title
Lot Number
343242
References
Reference overview
Caputo, C., and Bolanos, P. 1994. J. Physiol. (London)481, 119. Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134. Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun.163, 309.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Membrane-permeable version of FLUO 3 (Cat. No. 343244). Cleavage of FLUO 3/AM by cytosolic esterases traps the indicator in the cell. FLUO 3 is almost nonfluorescent at resting calcium levels, but fluorescence levels increase 40-fold upon calcium binding. Unlike all other Ca2+ indicators, FLUO 3/AM is nonfluorescent until it is hydrolyzed by the cell. Since its excitation maximum is at 506 nm, this compound can be used in conjunction with caged compounds, which are released by UV light. Its relatively high Kd for Ca2+ facilitates the measurement of higher peaks of Ca2+ transients than is possible with FURA 2. FLUO 3 has an excitation maximum of 506 nm and an emission maximum of 526 nm in the presence of high and low calcium concentrations.
Form
Reddish orange solid
Recommended reaction conditions
The FLUO 3/AM DMSO stock solution should be diluted to 1-5 µM in a physiological buffer. In some cases, use of the non-ionic detergent PLURIONIC® F-127, PROTEIN GRADE® (Cat. No. 540025) at 0.025-0.125% will assist in the dissolution of the dye in aqueous buffers. Cells are normally incubated with the dye for 20-60 min at 20-37°C.
CAS number
121714-22-5
Chemical formula
C₅₁H₅₀Cl₂N₂O₂₃
Structure formula
Purity
≥95% by HPLC
Solubility
DMF and DMSO (5 mM)
Storage
Protect from light -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Caputo, C., and Bolanos, P. 1994. J. Physiol. (London)481, 119. Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134. Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun.163, 309.