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330657 Estrogen Receptorβ, Human, Recombinant, S. frugiperda

Estrogen Receptor β, Human, Recombinant, S. frugiperda, supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
330657
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      Recombinant, human estrogen receptor-β expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that shares high homology with ER-α, especially in the DNA binding protein domain, suggesting that both receptors interact with related DNA response elements. Supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.
      Aliquot in individual vials for daily use. Do not add any dilution buffers, as these may cause aggregation of the ER.
      Catalogue Number330657
      Brand Family Calbiochem®
      References
      ReferencesKuiper, G.G., et al. 1997. Endocrinology 138, 863.
      Paech, K., et al. 1997. Science 277, 1508.
      Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
      Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of estrogen receptor that will bind 1.0 pmol ³H-estradiol overnight at 4°C as measured by quantitation of estradiol:receptor complexes in a hydroxylapatite assay.
      FormLiquid
      FormulationIn 400 mM KCl, 50 mM Bis-Tris-propane, 2 mM DTT, 1 mM EDTA, 10% glycerol, pH 9.0. DO NOT VORTEX.
      Biological Information
      Purity≥80% by SDS-PAGE
      Specific Activity≥5000 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not add any dilution buffers, as these may cause aggregation of the ER.
      Global Trade Item Number
      Catalogue Number GTIN
      330657 0