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1.15934

Eosin B (bluish) (C.I.45400)

for microscopy Certistain®

Synonym(s):

Eosin B (bluish) (C.I.45400), Dibromodinitrofluorescein sodium

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About This Item

Empirical Formula (Hill Notation):
C20H6Br2N2Na2O9
CAS Number:
548-24-3
Molecular Weight:
624.06
UNSPSC Code:
41116130
EC Index Number:
208-943-1
MDL number:
MFCD00005041
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Quality Segment

400

form

solid

composition

Dye, ≥85% spectrophotometric assay

pH

6.2 (25 °C, 10 g/L in H2O)

solubility

300 g/L

bulk density

680 kg/m3

εmax

800-1000 at 517-525 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Na+].[Na+].Brc1c2c(cc(c1[O-])[N+](=O)[O-])C(=C4C(=C(C(=O)C(=C4)[N+](=O)[O-])Br)O2)c3c(cccc3)C(=O)[O-]

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

General description

Eosin B (C.I. 45400) for microscopy Certistain®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear dark blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances pink to red. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (gravimetrically): ≥ 85 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 517 - 525 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 800 - 1000
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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