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324856 EGF, Murine Submaxillary Glands, Receptor Grade

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Epidermal Growth Factor

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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥98% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Native epidermal growth factor from mouse submaxillary glands. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Purified by size-exclusion chromatography followed by DEAE ion-exchange chromatography.
      Effective biological range: 5-50 ng/ml.
      Catalogue Number324856
      Brand Family Calbiochem®
      SynonymsEpidermal Growth Factor
      References
      ReferencesCanals, F., et al. 1992. Biochemistry 31, 4493.
      Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
      Sato, J., et al. 1987. Methods Enzymol. 146, 63.
      Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.
      Product Information
      CAS number62229-50-9
      FormLyophilized
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activitydetermined by mitogenic assay with human foreskin fibroblasts
      Purity≥98% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSKB4518000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      324856 0

      Documentation

      EGF, Murine Submaxillary Glands, Receptor Grade Certificates of Analysis

      TitleLot Number
      324856

      References

      Reference overview
      Canals, F., et al. 1992. Biochemistry 31, 4493.
      Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
      Sato, J., et al. 1987. Methods Enzymol. 146, 63.
      Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-May-2008 RFH
      SynonymsEpidermal Growth Factor
      DescriptionNative epidermal growth factor from mouse submaxillary glands. Purified by size-exclusion chromatography. Exhibits mitogenic activity in a wide range of tissues of ectodermal and endodermal origin. Effective at concentrations ranging from 5-50 ng/ml.
      FormLyophilized
      CAS number62229-50-9
      RTECSKB4518000
      Purity≥98% by SDS-PAGE
      Biological activitydetermined by mitogenic assay with human foreskin fibroblasts
      SolubilityReconstitute in culture medium, buffer, H₂O, or 100 mM acetic acid. Wipe the vial with 70% ethanol, add solvent, and swirl gently to dissolve.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 3527
      ReferencesCanals, F., et al. 1992. Biochemistry 31, 4493.
      Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
      Sato, J., et al. 1987. Methods Enzymol. 146, 63.
      Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors