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234590 Concanavalin A Plate, Clear Polystyrene

Concanavalin A Plate, Clear Polystyrene MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
234590
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Useful for the capture of glycoproteins or oligosaccharides containing terminal glucose or mannose residues. Suitable for colorimetric detection systems such as alkaline phosphatase or horseradish peroxidase. The physical and optical properties of the plate are not affected by the coupling chemistry. Data sheet included.
      Catalogue Number234590
      Brand Family Calbiochem®
      References
      ReferencesKobayashi, Y., et al. 1987. Immunology 60, 213.
      O'Shannessy, D.J., and Hofmann, W.L. 1987. Biotech. Appl. Biochem. 9, 488.
      Product Information
      FormCon A covalently bound to clear polystyrene plate
      Format96-well plate
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20/21/22-40-42/43

      Harmful by inhalation, in contact with skin and if swallowed.
      Limited evidence of a carcinogenic effect.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 36

      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful & Carcinogenic / Teratogenic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      234590 0

      Documentation

      Concanavalin A Plate, Clear Polystyrene Certificates of Analysis

      TitleLot Number
      234590

      References

      Reference overview
      Kobayashi, Y., et al. 1987. Immunology 60, 213.
      O'Shannessy, D.J., and Hofmann, W.L. 1987. Biotech. Appl. Biochem. 9, 488.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-June-2008 RFH
      DescriptionConcanavalin A (Con A), a lectin derived from the jack bean, has been used in a column format for the separation and purification of glycoproteins and carbohydrates based on the affinity of terminal mannose and glucose groups for Con A.2 Using a unique and proprietary surface chemistry procedure, our Con A plates (96 well) or strips (12 strips of 8 wells in a 96-well configuration) have the lectin covalently attached to the well surfaces. The physical and optical properties of the plate are unaffected by the coupling chemistry. The Con A plates and strips exhibit high affinity for terminal mannose and glucose groups and can be used to immobilize glycoproteins or carbohydrates containing these groups. The immobilized material can then be assayed with a standard ELISA system using tagged antibodies to the immobilized species.
      The plates or strips are available in clear or opaque white polystyrene. The opaque white polystyrene plates or
      strips can be used in fluorescent or chemiluminescent detection systems.
      Con A plates can also be used as a substrate for cell growth because of the affinity of the cell surface carbohydrate groups for Con A. It is possible to do cell panning, selecting those cells containing terminal mannose or glucose groups for immobilization; however, please note that these plates are not sterile. To use them for cell growth studies, it may be necessary to sterilize them. They can be safely sterilized either by ethanol or irradiation.
      FormCon A covalently bound to clear polystyrene plate
      Recommended reaction conditionsSuggested Protocol 1. Rehydrate the plate or strips by filling each well with buffer at neutral pH and allow to sit for 10 min. Rinse twice with buffer. 2. Fill the wells with the glycoprotein or carbohydrate in an appropriate buffer at a concentration of 1-3. µg/ml at neutral pH. The buffer should contain 1 mM Ca2+ and 1 mM Mn2+. 3. Allow binding to proceed for 2 h at 37°C. 4. Wash 3 times with buffer to remove unbound material and extra ions. 5. To use in an immunoassay, add a tagged antibody at 1-3 µg/ml in buffer and allow to react for 2 h or less at 37°C. The time depends on the speed of the antibody-antigen reaction. Typically, either horseradish peroxidase or alkaline phosphatase is used as tags. 6. Develop color using the appropriate substrate. For a usual assay, color development with alkaline phosphatase takes about an hour and horseradish peroxidase takes about 15 min.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Harmful & Carcinogenic / Teratogenic
      ReferencesKobayashi, Y., et al. 1987. Immunology 60, 213.
      O'Shannessy, D.J., and Hofmann, W.L. 1987. Biotech. Appl. Biochem. 9, 488.

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      Categories

      Life Science Research > Protein Sample Preparation > Protein Purification > Other Affinity Resins & Kits