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227036
Sigma-AldrichCholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free - CAS 9012-63-9 - Calbiochem
Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.
More>>Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.
Useful for tissue culture applications.
Catalogue Number
227036
Brand Family
Calbiochem®
References
References
Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.
Product Information
CAS number
9012-63-9
Activity
Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
ATP Competitive
N
Declaration
Not available for sale outside of the United States.
Form
Solid
Formulation
Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.
Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeable
N
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
LF3100000
Safety Information
R Phrase
R: 21/22-36/37/38
Harmful in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin.
S Phrase
S: 7-36/37
Keep container tightly closed. Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Highly Toxic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+2°C to +8°C
Do not freeze
Yes
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
End use certificate
Y
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number
GTIN
227036-1MG
04055977200560
Documentation
Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free - CAS 9012-63-9 - Calbiochem SDS
Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free - CAS 9012-63-9 - Calbiochem Certificates of Analysis
Title
Lot Number
227036
References
Reference overview
Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-March-2024 JSW
Description
Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..
Form
Solid
Formulation
Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
CAS number
9012-63-9
RTECS
LF3100000
Purity
≥95% by SDS-PAGE
Activity
Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
Solubility
Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.
Storage
+2°C to +8°C
Do Not Freeze
Yes
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
