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HTS014M ChemiSCREEN™ CCR10 Membrane Preparation

Human CCR10 GPCR membrane preparation for GTPγS Binding Assays.

ChemiSCREEN™ CCR10 Membrane Preparation MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P51686 Entrez Gene Number: AF215981
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HTS014M
200 assays  
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      Overview

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1CCR10
      Description
      Catalogue NumberHTS014M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ CCR10 Membrane Preparation
      OverviewFull length human CCR10 with a proprietary mutation in the cytoplasmic domain to enhance cell surface expression.
      Background InformationCCR10, previously known as GPR2, is a GPCR that is expressed in dermal endothelial cells, fibroblasts, melanocytes, and circulating T cells and PBMCs (Homey et al., 2000). Two chemokines, CTACK/CCL27 and MEC/CCL28, bind to CCR10 and stimulate migration and calcium flux (Pan et al., 2000; Wang et al., 2000). Expression of CTACK by keratinocytes, and CCR10 by lymphocytes from skin of patients with psoriasis and dermatitis, implicates this receptor ligand pair in skin inflammation. In addition, disruption of CCL27-CCR10 interaction reduces skin inflammation induced by allergen (Homey et al., 2002). CCR10 expressed on IgA antibody-secreting cells also mediates their migration to CCL28 expressed in the lactating mammary gland, and subsequent IgA accumulation in milk (Wilson and Butcher, 2004). Millipore's CCR10 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR10 interactions with its ligands. The membrane preparations exhibit an EC50 of 47 nM for CTACK/CCL27 in a GTPγS binding assay.
      Product Information
      FormatMembranes
      PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.

      Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
      Quality LevelMQ100
      Applications
      ApplicationHuman CCR10 GPCR membrane preparation for GTPγS Binding Assays.
      Key Applications
      • GTPγS binding
      Application NotesGTPγS Binding Assay.
      Biological Information
      Concentration1.0 mg/mL
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
      Gene Symbol
      • CCR9
      • CC-CKR-9
      • D6
      • GPR-9-6
      • CDw199
      • CCBP2
      • GPR28
      • CCR10
      • CCR-9
      • CMKBR9
      GPCR ClassA
      Host CellsChem-1
      Protein TargetCCR10
      Target Sub-FamilyChemokine
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P51686 # Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
      SIZE: 369 amino acids; 42016 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Highly expressed in the thymus and low in lymph nodes and spleen.
      SIMILARITY: SwissProt: P51686 ## Belongs to the G-protein coupled receptor 1 family.
      MISCELLANEOUS: EC50 of SCYA25/TECK for isoform 1 is lower than for isoform 2.
      Incubation ConditionsGTPγS ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled ligand was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
      Product Usage Statements
      Quality AssuranceEC50 for CTACK/CCL27: ~5.6 nM in a GTPγS binding assay
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
      Packaging Information
      Material Size200 assays
      Global Trade Item Number
      Catalogue Number GTIN
      HTS014M 04053252329760