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218769 Caspase-8, Human, Recombinant, E. coli

Caspase-8, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FLICE, MACH, Mch5

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218769
  
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      Overview

      Replacement Information
      Description
      OverviewRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
      M.W. 11,000 and 18,000 (heterodimer).
      Catalogue Number218769
      Brand Family Calbiochem®
      SynonymsFLICE, MACH, Mch5
      References
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
      Muzio, M., et al. 1996. Cell 85, 817.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      218769 0

      Documentation

      Caspase-8, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Caspase-8, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218769

      References

      Reference overview
      Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
      Muzio, M., et al. 1996. Cell 85, 817.

      Citations

      Title
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
    • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-September-2007 RFH
      SynonymsFLICE, MACH, Mch5
      DescriptionRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Purity≥90% by SDS-PAGE
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (Cat. No. 368057) per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
      Toxicity Standard Handling
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
      Muzio, M., et al. 1996. Cell 85, 817.
      Citation
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
    • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
      		•