Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
A simple and rapid method for the estimation of protein concentration. Based on an improved Coomassie dye reagent. Reaches a stable endpoint in 5 minutes. Compatible with reducing reagents, but not suitable for use with solutions containing detergents.
Catalogue Number
219468
Brand Family
Calbiochem®
Application Data
Materials Required but Not Delivered
• Disposable polystyrene cuvettes • Assay tubes
References
Product Information
Form
500 tube assays or 5000 microwell plate assays
Format
cuvette or 96-well plate
Kit contains
CB-Protein Dye, Albumin Standard, and a user protocol.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 36/37/38
Irritating to eyes, respiratory system and skin.
S Phrase
S: 26-36
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Storage
+2°C to +8°C
Storage Conditions
Upon arrival store entire contents of the kit at 4°C.
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
CB-Protein Dye, Albumin Standard, and a user protocol.
Upon arrival store entire contents of the kit at 4°C.
Principles of the assay
The CB-Protein Assay™ Kit offers an improvement over the well-known Bradford Coomassie dye-binding assay. This assay is based on a single Coomassie dye reagent. In an acidic medium, protein binds with Coomassie dye and produces a blue protein-dye complex that can be measured at 595 nm. The test can be performed using as little as 1 µl protein per sample.
Protein + Coomassie in acidic medium → Blue Color Protein-Dye Complex
Standard Curve for the CB-Protein Assay™ Kit shows a substantially linear relationship between absorbance by density of protein-dye complex and the protein concentration.
Materials provided
• CB-Protein Assay™ Reagent (Kit Component No. KP15201): 1 x 500 ml • Bovine Serum Albumin Standard (2 mg/ml) (Kit Component No. KP15202): 1 x 5 ml
Materials Required but not provided
• Disposable polystyrene cuvettes • Assay tubes
Precautions and recommendations
• Allow the CB-Protein Assay™ Reagent to warm to room temperature. Prior to use, mix the reagent gently by inverting the bottle several times. DO NOT SHAKE THE BOTTLE. • Staining of glassware: clean glassware with methanol. • Effect of temperature: consistent results are obtained when the CB-Protein Assay™ Kit is at room temperature. Allow components to warm to room temperature prior to use.
Preparation
• Preparation of diluted protein standard: For minimizing interference, it is important to prepare the appropriate diluted protein standard in the same diluent used for the test protein sample.
Standard Protocol (2000-25 µg protein/ml)
Table 1: Standard Protocol
Micro Protocol Prepare a 100 µg protein/ml stock solution by mixing 25 µl (2 mg/ml stock) albumin solution and 475 µl diluent. Use this stock for preparing diluted protein standard for the micro protocol assay. For minimizing interference, it is important to prepare the standard in the same diluent used for the test protein sample.
Table 2: Micro Protocol
Detailed protocol
Protein assay can be performed either by the standard protocol in the concentration range 100-1,000 µg protein/ml BSA or by micro-assay protocol for dilute protein solution in the working range of 1-25 µg/ml.
Standard - Test Tube Assay: (Protein Concentration Range 100-1000 µg/ml)
Perform assay with duplicate protein standards and samples. Read Precautions Recommendations. 1. Transfer 0.05 ml of diluted standards and test samples in the assay tubes. Set up a blank containing 0.05 ml of protein solution diluent. 2. Add 1 ml of CB-Protein Assay™ reagent into each tube and mix well. Allow the tubes to incubate at room temperature for at least 5 min, but no more than 1 h. 3. Read absorbance of the assay tubes at 595 nm. 4. Subtract reading of the blank with the test samples and plot a standard curve for determination of the protein concentration of unknown samples.
Standard - 96-Well Plate Assay: (Protein Concentration Range 100-1000 µg/ml)
Perform assay with duplicate protein standards and samples. Read "Preparation Before Use". 1. Transfer 10 µl of diluted standards and test samples into plate wells. Set up a blank containing 10 µl of protein solution diluent. 2. Add 200 µl of CB-Protein Assay™ reagent into each well and mix well. Allow the wells to incubate at room temperature for at least 5 min, but no more than 1 h. 3. Read absorbance of the assay tubes at 595 nm. 4. Subtract reading of the blank with the test samples and plot a standard curve for determination of the protein concentration of unknown samples. Micro Protocol: Test Tube Assay (Protein Conentration Range 1-25 µg/ml)
Perform assay with duplicate protein standards and samples. Read Precautions Recommendations. 1. Transfer 0.5 ml of diluted standards and test samples in the assay tubes. Set up a blank containing 0.5 ml of protein solution diluent. 2. Add 0.5 ml of CB-Protein Assay™ reagent into each tube and mix well. Allow the tubes to incubate at room temperature for at least 5 min, but no more than 1 h. 3. Read absorbacne of the assay tubes at 595 nm. 4. Subtract reading of the blank with the test samples and plot a standard curve for determination of the protein concentration of unknown samples.
Micro Protocol: Plate Assay (Protein Concentration Range 1-25 µg/ml)
Perform assay with duplicate protein standards and samples. Read Precautions Recommendations. 1. Transfer 100 µl of diluted standards and test samples into plate wells. Set up a blank containing 100 µl of protein solution diluent. 2. Add 100 µl of CB-Protein Assay™ reagent into each well and mix well. Allow the wells to incubate at room temperature for at least 5 min, but no more than 1 h. 3. Read absorbance of the assay tubes at 595 nm. 4. Subtract reading of the blank with the test samples and plot a standard curve for determination of the protein concentration of unknown samples.
Registered Trademarks
Calbiochem® is a registered trademark of EMD Chemicals, Inc. Triton® is a registered trademark of Dow Chemical Company Tween® and Brij® are registered trademarks of ICI Americas, Inc. Interactive Pathways™ is a trademark of EMD Chemicals, Inc. CB-Protein Assay™ Kit is a trademark of Geno Technology, Inc.
