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SCT238 BioTracker Apo-15 Calcium-Independent Apoptosis Probe

Green fluorescent probe for detecting early stage apoptosis in live cells by microscopy or flow cytometry.

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SCT238
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT238
      DescriptionBioTracker Apo-15 Calcium-Independent Apoptosis Probe
      OverviewThe BioTracker Apo-15 Calcium-Independent Apoptosis Probe is a calcium-independent, “turn-on” fluorescence probe emitting in the green spectrum for imaging and detection of apoptotic live cell under wash-free conditions. Like Annexin V, Apo-15 binds phosphatidylserine (PS) during early stage apoptosis when PS is translocated to the outer plasma membrane (Barth, 2020).

      Apoptosis is dysregulated in numerous diseases, from inflammatory conditions to cancer. Detection of apoptosis is a key output in assessing drug efficacy, yet the low levels of free calcium in diseased tissues limit use of annexins as apoptosis detection reagents, favoring calcium-independent fluorogenic detection. Apo-15 binds negatively-charged phospholipids exposed on apoptotic cell membranes without interfering with cellular function. Apo-15 is non-fluorescent in viable cells but highly fluorescent in apoptotic cells, enabling highly specific detection of early apoptosis. Apoptotic imaging and detection with Apo-15 is broadly applicable to a wide range of cell types and experimental conditions.

      Spectral Properties
      Fluorescence images obtained by λex = 500 nm and emission at 520 nm.

      Reference
      Barth, Nicole D., et al. "A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis." Nature communications 11.1 (2020): 1-14.
      Alternate Names
      • Live Cell Imaging Probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized. Orange solid.
      Quality LevelMQ100
      Applications
      ApplicationGreen fluorescent probe for detecting early stage apoptosis in live cells by microscopy or flow cytometry.
      Key Applications
      • Cell Based Assays
      • Apoptosis Assay
      Application NotesLive cell fluorescent imaging
      Biological Information
      Molecular Weight1311.33
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity ≥ 98% confirmed by HPLC. Identification confirmed by H NMR, LC-MS, and elemental analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C, desiccate and protect from light.
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT238 04065267532202

      Documentation