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ABE1392

Anti-phospho SIRT1 (Ser682) Antibody

Name: Anti-phospho SIRT1 (Ser682) Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, phospho SIRT1 (Ser682)

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

polyclonal

Species reactivity:

human

Application:

Citations:

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Properties

biological source

rabbit

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

feline (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_036370

UniProt accession no.

Q96EB6

shipped in

dry ice

target post-translational modification

phosphorylation (pSer682 )

Gene Information

human ... SIRT1(23411)

Description

General description

NAD-dependent protein deacetylase sirtuin-1 (UniProt Q96EB6; also known as hSIR2, hSIRT1, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha, Sirtuin type 1) is encoded by the SIRT1 (also known as SIR2L1) gene (Gene ID 23411) in human. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms studied. Sirtuins have been implicated in the regulation of multiple processes, including aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) and aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 and pS47 targeted by CaMKKβ.

~110 kDa observed. The target band size appears larger than the calculated molecular weight (81.7 kDa/isoform 1 & 61.1 kDa/isoform 2). Uncharacterized band(s) may appear in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human phospho SIRT1 (Ser682) near the C-terminus.

Application

Anti-phospho SIRT1 (Ser682) Antibody, Cat. No. ABE1392, is a highly specific rabbit Polyclonal antibody, that targets SIRT1 and has been tested in Western Blotting.

Biochem/physiol Actions

Ser682 of hSIRT1 isoform 1 is equivalent to Ser496 of hSIRT1 spliced isoform 2 (delta-exon8).

Analysis Note

Evaluated by Western Blotting in Nocodazole treated H1299 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho SIRT1 (Ser682) in 10 µg of Nocodazole treated cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c