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ABC1414-25UG Anti-phospho IGPR-1 (Ser220)

Anti-phospho IGPR-1 (Ser220), Cat. No. ABC1414, is a rabbit polyclonal antibody that detects IGPR-1 phosphorylated on serine 220 and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

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ABC1414-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC1414-25UG
      DescriptionAnti-phospho IGPR-1 (Ser220)
      Alternate Names
      • Transmembrane and immunoglobulin domain-containing protein 2
      • CD28 homolog
      • Immunoglobulin and proline-rich receptor 1
      Background InformationTransmembrane and immunoglobulin domain-containing protein 2 (UniProt: Q96BF3; also known as CD28 homolog, Immunoglobulin and proline-rich receptor 1, IGPR-1) is encoded by the TMIGD2 (also known as CD28H, IGPR1, UNQ3059/PRO9879) gene (Gene ID: 126259) in human. IGPR-1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-150), a transmembrane domain (aa 151-171), and a cytoplasmic domain (aa 172-282). It is widely expressed with higher expression observed in epithelial and endothelial cells, including bronchial epithelial cells of lung, breast glandular and lobular epithelia cells. It plays a role in cell-cell interaction, cell migration, and angiogenesis. IGPR-1 is localized to endothelial adherens junctions, and through trans-homophilic dimerization it regulates endothelial cell-cell adhesion and barrier function. This dimerization stimulates phosphorylation of serine 220, which is required for IGPR-1 to regulate endothelial barrier function and angiogenesis. Through interaction with HHLA2, IGPR-1 is reported to co-stimulate T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an Akt-dependent signaling cascade. IGPR-1 is also phosphorylated on serine 220 by I B kinase β, which leads to its activation. Activated IGPR-1 then stimulates phosphorylation of AMPK, which in turn phosphorylates ULK1 and Beclin-1 and leads to increased levels of LC3-II. Genotoxic agents such as doxorubicin and flow shear stress are also reported to simulate phosphorylation of IGPR-1 at serine 220. (Ref.: Amraei, R., et al. (2020). J. Biol. Chem. 295(49); 16691-16699; Wang, YHW., et al.(2016). J. Mol. Biol. 428(24; PtB); 5019-5033).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho IGPR-1 (Ser220), Cat. No. ABC1414, is a rabbit polyclonal antibody that detects IGPR-1 phosphorylated on serine 220 and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesTested applications

      Immunofluorescence Analysis: A representative lot detected phospho-IGPR-1 (Ser220) in Immunofluorescence applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

      Immunohistochemistry Applications: A representative lot detected phospho-IGPR-1 (Ser220) in Immunohistochemistry applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids surrounding phosphoserine 220 in the cytoplasmic domain in Human Immunoglobulin and proline-rich receptor 1 (IGPR-1).
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Immunoglobulin and proline-rich receptor 1 (IGPR-1) phosphorylated on serine 220.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TMIGD2
      Modifications
      • Phosphorylation
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~45 to 55 kDa observed; 30.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells overexpressing IGPR-1.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected IGPR-1 phosphorylated on serine 220 in lysates from HEK293 cells overexpressing IGPR-1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1414-25UG 04065268979044