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ABN991 Anti-phospho GLUT-1 Antibody (Ser226)

This Anti-phospho GLUT-1 Antibody (Ser226) is validated for use in Western Blotting, Peptide Inhibition Assay, Immunocytochemistry for the detection of phospho GLUT-1.

Anti-phospho GLUT-1 Antibody (Ser226) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: DYT9, DYT17, DYT18, EIG12, GLUT, GLUT1, GLUT1DS, HTLVR, PED, SLC2A1 UniProt Number: P11166
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ABN991
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, PIA, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN991
      DescriptionAnti-phospho GLUT-1 Antibody (Ser226)
      Alternate Names
      • Solute carrier family 2, facilitated glucose transporter member 1
      • Glucose transporter type 1, erythrocyte/brain
      • GLUT-1
      • HepG2 glucose transporter
      • Human T-cell leukemia virus I and II receptor
      • Receptor for HTLV-1 and HTLV-2
      • phospho GLUT-1 (Ser226)
      Background InformationSolute carrier family 2, facilitated glucose transporter member 1 (UniProt P11166; also known as Glucose transporter type 1 erythrocyte/brain, GLUT-1, HepG2 glucose transporter, Human T-cell leukemia virus I and II receptor, Receptor for HTLV-1 and HTLV-2) is encoded by the SLC2A1 (also known as DYT9, DYT17, DYT18, EIG12, GLUT, GLUT1, HTLVR, GLUT1DS, PED) gene (Gene ID 6513) in human. Glucose transporters (GLUT-1 to GLUT-7) constitute a family of integral membrane glycoproteins that facilitate cellular glucose uptake. In addition to mediating glucose uptake in adult tissues, GLUT-1 is the predominant glucose transporter in embryonic and fetal tissues. Two forms of GLUT-1 are found in human frontal cortex, the 55 kDa form of GLUT-1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT-1 predominantly regulates nonvascular glial glucose uptake.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho GLUT-1 Antibody (Ser226) is validated for use in Western Blotting, Peptide Inhibition Assay, Immunocytochemistry for the detection of phospho GLUT-1.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      • Immunocytochemistry
      Application NotesPeptide Inhibition Analysis: 2.0 µg/mL from a representative lot detected Ser226 phosphorylated GLUT-1 in lysates from PMA-treated HeLa cells. Pre-incubation of the antibody with the immunogen peptide, but not the correspoding non-phosphorylated peptide, blocked the detection of the phospho GLUT-1 band.
      Western Blotting Analysis: An 1:200 dilution (5 µg/mL) from a representative lot detected TPA-induced phosphorylation of wild-type GLUT-1, but not GLUT-1 with S226A mution in lysates from Rat2 fibroblasts expsssing the respective constructs via retrovirus-mediated transfection (Courtesy of Dr. Richard C. Wang, UT Southwestern Medical Center, Dallas, TX).
      Western Blotting Analysis: An 1:200 dilution (5 µg/mL) from a representative lot detected a time-dependent GLUT-1 Ser226 phosphorylation induction in serum-starved human umbilical vein endothelial cells (HUVECs) and human aortic endothelial cells (HAECs) upon VEGF treatment (Courtesy of Dr. Richard C. Wang, UT Southwestern Medical Center, Dallas, TX).
      Western Blotting Analysis: A representative lot detected PKC activation-induced GLUT-1 Ser226 phosphorylation in human aortic endothelial cells (HAECs) upon TPA (Cat. No. 500582 & 524400) treatment only the in the absence, but not in the presence, of PKC inhibitor Go 6983 (Cat. No. 365251) (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Western Blotting Analysis: A representative lot detected a time-dependent GLUT-1 Ser226 phosphorylation induction in serum-starved human umbilical vein endothelial cells (HUVECs) upon VEGF or angiotensin II treatment (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Western Blotting Analysis: A representative lot detected comparable Ser226 phosphorylation induction of exogenously expressed wild-type GLUT-1 or K526E mutant in transfected Rat2 fibroblasts upon PKC activator TPA (Cat. No. 500582 & 524400) treatment (Cat. No. 365251) (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Western Blotting Analysis: A representative lot detected PKC activator TPA-induced GLUT-1 Ser226 phosphorylation in serum-starved HeLa, human primary cardiac endothelial cells, EA.hy926 human endothelial cells, and bEnd.3 mouse brain endothelial cells (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Western Blotting Analysis: A representative lot detected PKC-catalyzed Ser226 phosphorylation of GST fusion protein containing wild-type GLUT-1 Loop 6 (4th cytoplasmic domain) seuqnce, but not GST-Loop 6 fusions with R223P, R223Q, R223W, or S226A mutation, in in vitro kinase assays (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Western Blotting Analysis: A representative lot detected a time-dependent GLUT-1 Ser226 phosphorylation induction in serum-starved human aortic endothelial cells (HAECs) upon VEGF treatment (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Immunocytochemistry Analysis: A representative lot detected PKC activation-induced GLUT-1 Ser226 phosphorylation in the membrane ruffles of human umbilical vein endothelial cells (HUVECs) upon TPA (Cat. No. 500582 & 524400) treatment (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
      Note: Process lysate samples by warming at 50°C for 10 minutes prior to gel loading. Avoid heating samples at a temperature higher than 60°C, which can cause aggregation of the target protein.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a fourth cytoplasmic domain (Loop 6) sequence of human GLUT-1 with phosphorylated Ser226.
      EpitopepSer226 in the fourth cytoplasmic domain (Loop 6).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Canine, Xenopus, Rabbit, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DYT9
      • DYT17
      • DYT18
      • EIG12
      • GLUT
      • GLUT1
      • GLUT1DS
      • HTLVR
      • PED
      • SLC2A1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~50 kDa observed. A smeared banding pattern between 45-75 kDa can often be seen, representing target bands with varying degrees of glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Ser226 phosphorylated GLUT-1 in lysates from PMA-treated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN991 04055977312867

      Documentation

      Anti-phospho GLUT-1 Antibody (Ser226) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho GLUT-1 Antibody (Ser226) Certificates of Analysis

      TitleLot Number
      Anti-phospho GLUT-1 (Ser226) - 3740920 3740920
      Anti-phospho GLUT-1 (Ser226) - 3277973 3277973
      Anti-phospho GLUT-1 (Ser226) - 3426909 3426909
      Anti-phospho GLUT-1 (Ser226) - 3837014 3837014
      Anti-phospho GLUT-1 (Ser226) - 3954749 3954749
      Anti-phospho GLUT-1 (Ser226) - 4163758 4163758
      Anti-phospho GLUT-1 (Ser226) -2819306 2819306
      Anti-phospho GLUT-1 (Ser226) -Q2639565 Q2639565
      Anti-phospho GLUT-1 (Ser226) Polyclonal Antibody 3150366
      Anti-phospho GLUT-1 (Ser226) Polyclonal Antibody 3030537
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