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MABE1076-25UG Anti-mono ADP Ribose Binding Reagent

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MABE1076-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, DBE. coliPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1076-25UG
      DescriptionAnti-mono ADP Ribose Binding Reagent
      Background InformationAnti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
      References
      Product Information
      FormatPurified
      PresentationPurified E. coli recombinant monoclonal binding reagent in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM beta-Mercaptoethanol without preservatives with 10% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-mono ADP Ribose Binding Reagent Antibody, Cat. No. MABE1076-25UG, validated for use in Dot Blot and Western Blotting.
      Key Applications
      • Western Blotting
      • Dot Blot
      Application NotesWestern Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins.
      Biological Information
      ConcentrationPlease refer to lot specific datasheet.
      HostE. coli
      SpecificityThis reagent binds to mono-ADP ribosylated proteins.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteMouse, Human.
      Antibody TypeMonoclonal Antibody
      Purification MethodImmobilized Metal Affinity Chromatography (IMAC)
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Gel Electrophoresis. Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1076-25UG 04054839420900

      Documentation

      Anti-mono ADP Ribose Binding Reagent SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-mono ADP Ribose Binding Reagent Certificates of Analysis

      TitleLot Number
      Anti-mono ADP Ribose Binding Reagent - 3172396 3172396
      Anti-mono ADP Ribose Binding Reagent - 3291274 3291274
      Anti-mono ADP Ribose Binding Reagent - 3388444 3388444
      Anti-mono ADP Ribose Binding Reagent - 3403480 3403480
      Anti-mono ADP Ribose Binding Reagent - 3458521 3458521
      Anti-mono ADP Ribose Binding Reagent - 3489738 3489738
      Anti-mono ADP Ribose Binding Reagent - 3501839 3501839
      Anti-mono ADP Ribose Binding Reagent - 3551476 3551476
      Anti-mono ADP Ribose Binding Reagent - 3601804 3601804
      Anti-mono ADP Ribose Binding Reagent - 3677790 3677790