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MABS1280-25UG Anti-mS2i6A Antibody, clone CL-6

Anti-mS2i6A, clone CL-6 Antibody, Cat. No. MABS1280-25UG, validated for use in Dot Blot and ELISA.

Research Category: Epigenetics & Nuclear Function UniProt Number: N/A
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MABS1280-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MaDB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1280-25UG
      DescriptionAnti-mS2i6A Antibody, clone CL-6
      Alternate Names
      • N6-isopenetyl-2-thiomethyladenosine
      Background InformationThe mS2i6A is an evolutionally conserved modification found in the tRNA. The hypermodified mS2i6A at position 37 occurs frequently in tRNAs that read codons starting with uridine. The mS2 group of mS2i6A is critical for the accurate decoding of Tyr and Phe codons. It has been shown that a deficiency of mS2i6A in the cognate poly(U) reader tRNA(Phe) leads to increased misreading when the near-cognate competitor tRNA4(Leu) is wild-type. It can lead to higher rejection rate of tRNA4(Leu) by more aggressive proofreading on the wild-type ribosome. It is believed that Cdk5rap1 specifically catalyzes the mS2 modification of mt-tRNAs and contributes to mitochondrial function in vivo. This modification regulates efficient mitochondrial translation and energy metabolism in mammals. Cdk5rap1 knockout mice that are deficient in the 2-methylthio modification in mt-tRNAs bearing Ser(UCN), Phe, Tyr and Trp, impaired mitochondrial protein synthesis is observed, which causes respiratory defects of mitochondria and myopathy in vivo. (Ref.: Deuchler, M et al. (2016). Cell Mol Life Sci. 73 (16); 3075-3095; Fakruddin, M., et al. (2017). Nucleic Acids Res. 2017 Nov 16;45(20):11954-11961).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-mS2i6A, clone CL-6 Antibody, Cat. No. MABS1280-25UG, validated for use in Dot Blot and ELISA.
      Key Applications
      • Dot Blot
      • ELISA
      Application NotesDot Blot Analysis: A 1:200 dilution of this antibody detected mS2i6A in Mouse liver total RNA and Cdk5rap1 KO  mouse total RNA (Courtesy of Fan - Yan Wei, Ph.D ., Faculty of Life Sciences,  Kumamoto University Japan ).
      Biological Information
      ImmunogenA synthesized mS2i6A nucleotide conjugated to BSA.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CL-6 recognizes wild-type total RNA, but not Cdk5rap1 KO  total RNA.
      IsotypeIgG2b
      Species Reactivity
      • Mammals
      Species Reactivity NoteMammals.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in Mouse Liver Total RNA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1280-25UG 04054839421082

      Documentation