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ST1200
Sigma-AldrichAnti-Ubiquitin Mouse mAb (FK2)
This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
More>>This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Less<<
Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Catalogue Number
ST1200
Brand Family
Calbiochem®
Application Data
Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
References
References
Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
ELISA
Takada, K., et al. 1995. Eur. J. Biochem.233, 42.
Takada, K., et al. 1997. Clin. chem.43, 1188.
Immunoprecipitation
Takada, K., et al. 2001. Biochem. J.356, 199.
Boname, J.N. and Stevenson, P.G. 2001. Immunity15, 627.
Matsumoto, M., et al. 2005. Proteomics5, 4145.
Immunofluorescence
Campbell, D.S. and Holt, C.E. 2001. Neuron32, 1013.
ELISA (see application references) Immunoblotting (1:1000) Immunoprecipitation (see application references and comments) Immunofluorescence (see application references)
Application Comments
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
unpurified polyubiquitinylated lysozyme
Immunogen
human
Clone
FK2
Host
Mouse
Isotype
IgG₁
Source
Raji cells
Species Reactivity
A Broad Range Of Species
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Anti-Ubiquitin Mouse mAb (FK2) Certificates of Analysis
Title
Lot Number
ST1200
References
Reference overview
Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-March-2009 JSW
Application
ELISA (see application references) Immunoblotting (1:1000) Immunoprecipitation (see application references and comments) Immunofluorescence (see application references)
Application Data
Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
Description
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
Host
Mouse
Immunogen species
human
Immunogen
unpurified polyubiquitinylated lysozyme
Clone
FK2
Isotype
IgG₁
Species
a broad range of species
Form
Liquid
Formulation
In PBS.
Concentration Label
Please refer to vial label for lot-specific concentration
Source
Raji cells
Preservative
≤0.1% sodium azide
Comments
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Toxicity
Standard Handling
References
Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Application references
ELISA
Takada, K., et al. 1995. Eur. J. Biochem.233, 42.
Takada, K., et al. 1997. Clin. chem.43, 1188.
Immunoprecipitation
Takada, K., et al. 2001. Biochem. J.356, 199.
Boname, J.N. and Stevenson, P.G. 2001. Immunity15, 627.
Matsumoto, M., et al. 2005. Proteomics5, 4145.
Immunofluorescence
Campbell, D.S. and Holt, C.E. 2001. Neuron32, 1013.